中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2010年
7期
524-527
,共4页
李疆%王国斌%孙仁虎%陶凯雄
李疆%王國斌%孫仁虎%陶凱雄
리강%왕국빈%손인호%도개웅
白细胞介素-1β%肠上皮化生%尾型同源盒转录因子2
白細胞介素-1β%腸上皮化生%尾型同源盒轉錄因子2
백세포개소-1β%장상피화생%미형동원합전록인자2
Interleukin-1 beta%Intestinal metaplasia%Caudal-related homeobox transcription factor 2
目的 探讨白介素(IL)-1β对人胃黏膜上皮细胞尾型同源盒转录因子2(CDX2)表达的调控及其在胃黏膜肠上皮化生中的作用.方法 用不同浓度IL-1β刺激人胃黏膜上皮GES-1细胞,采用实时荧光定量PCR法、免疫细胞化学染色法及免疫印迹方法,在不同时间点检测CDX2 mRNA和蛋白表达水平.使用NF-κB抑制剂二硫代氨基甲酸吡咯烷(PDTC)预处理GES-1细胞,观察PDTC对IL-1β诱导的CDX2 mRNA和蛋白表达的影响.电镜观察IL-1β长期刺激下GES-1细胞超微结构的改变.结果 IL-1β可以诱导人胃黏膜上皮细胞上调CDX2 mRNA的表达.予1μg/LIL-1β刺激后,GES-1细胞CDX2 mRNA和蛋白表达水平分别为0.0749±0.0021和0.56±0.04;加入PDTC后,CDX2 mRNA和蛋白表达水平分别为0.0006±0.0002和0.40±0.06;差异均有统计学意义(均P＜0.05).IL-1β长期刺激后,GES-1细胞表面会长出很多较长的微绒毛,同时细胞质内的分泌小泡也相应增加,胃上皮细胞开始出现肠上皮细胞的特征.结论 IL-1β能够通过NF-κB通路促进人胃黏膜上皮细胞CDX2 mRNA和蛋白表达上调,在胃黏膜肠上皮化生中发挥重要作用.
目的 探討白介素(IL)-1β對人胃黏膜上皮細胞尾型同源盒轉錄因子2(CDX2)錶達的調控及其在胃黏膜腸上皮化生中的作用.方法 用不同濃度IL-1β刺激人胃黏膜上皮GES-1細胞,採用實時熒光定量PCR法、免疫細胞化學染色法及免疫印跡方法,在不同時間點檢測CDX2 mRNA和蛋白錶達水平.使用NF-κB抑製劑二硫代氨基甲痠吡咯烷(PDTC)預處理GES-1細胞,觀察PDTC對IL-1β誘導的CDX2 mRNA和蛋白錶達的影響.電鏡觀察IL-1β長期刺激下GES-1細胞超微結構的改變.結果 IL-1β可以誘導人胃黏膜上皮細胞上調CDX2 mRNA的錶達.予1μg/LIL-1β刺激後,GES-1細胞CDX2 mRNA和蛋白錶達水平分彆為0.0749±0.0021和0.56±0.04;加入PDTC後,CDX2 mRNA和蛋白錶達水平分彆為0.0006±0.0002和0.40±0.06;差異均有統計學意義(均P＜0.05).IL-1β長期刺激後,GES-1細胞錶麵會長齣很多較長的微絨毛,同時細胞質內的分泌小泡也相應增加,胃上皮細胞開始齣現腸上皮細胞的特徵.結論 IL-1β能夠通過NF-κB通路促進人胃黏膜上皮細胞CDX2 mRNA和蛋白錶達上調,在胃黏膜腸上皮化生中髮揮重要作用.
목적 탐토백개소(IL)-1β대인위점막상피세포미형동원합전록인자2(CDX2)표체적조공급기재위점막장상피화생중적작용.방법 용불동농도IL-1β자격인위점막상피GES-1세포,채용실시형광정량PCR법、면역세포화학염색법급면역인적방법,재불동시간점검측CDX2 mRNA화단백표체수평.사용NF-κB억제제이류대안기갑산필각완(PDTC)예처리GES-1세포,관찰PDTC대IL-1β유도적CDX2 mRNA화단백표체적영향.전경관찰IL-1β장기자격하GES-1세포초미결구적개변.결과 IL-1β가이유도인위점막상피세포상조CDX2 mRNA적표체.여1μg/LIL-1β자격후,GES-1세포CDX2 mRNA화단백표체수평분별위0.0749±0.0021화0.56±0.04;가입PDTC후,CDX2 mRNA화단백표체수평분별위0.0006±0.0002화0.40±0.06;차이균유통계학의의(균P＜0.05).IL-1β장기자격후,GES-1세포표면회장출흔다교장적미융모,동시세포질내적분비소포야상응증가,위상피세포개시출현장상피세포적특정.결론 IL-1β능구통과NF-κB통로촉진인위점막상피세포CDX2 mRNA화단백표체상조,재위점막장상피화생중발휘중요작용.
Objective To evaluate the effect of IL-lβ on the expression of CDX2 in human gastric epithelial cell line CES-1 and its role in the intestinal metaplasia. Methods GES-1 cells were treated with IL-ip in different concentrations and the expressions of CDX2 mRNA and protein were detected by real-time PCR, immunocytochemistry and Western blot at different time points. GES-1 cells were then pre-treated with NF-KB pathway inhibitor PDTC, and the expression of CDX2 mRNA and protein induced by IL-1β were detected. The cell ultra-structure of GES-1 cells was observed by electronic microscope after GES-1 being treated with IL-1β for 25 days. Results Levels of CDX2 mRNA and protein were 0.0749±0.0021 and 0.56±0.04 in the cells treated with 1 μg/L IL-1β (P< 0.05). After pre-treatment with PDTC, levels of CDX2 mRNA and protein were 0.0006±0.0002 and 0.40 ±0.06 (P<0.05). Some changes in the cell ultra-structure of GES-1 were found by electronic microscope when GES-1 was treated with IL-1β for 25 days. Conclusion IL-1β can stimulate CDX2 mRNA and protein expression in GES-1 cells through the NF-KB signal pathway, indicating that IL-1β plays an important role in the intestinal metaplasia.
