CAJ | 학술논문

目的探讨乙型肝炎病毒表面大蛋白(LHBs)在监测抗病毒疗效上的临床意义.方法跟踪测46例阿德福韦酯治疗患者60周,分别采用ELISA法、时间分辨免疫荧光分析法和实时定量PCR法检测患者不同时间的LHBs、HBV血清学标志物和HBV DNA载量,并进行相关性分析.结果 LHBs和HBV DNA下降趋势一致,其相关系数r=0.97,但LHBs消退晚于HBV DNA.治疗60周时共有20例患者的HBV DNA<5×102/mL,其中8例LHBs转阴;疗效反复组14例患者HBVDNA转阴后又转阳,3例患者LHBs转阴;治疗无效组12例患者,2例LHBs转阴.结论动态监测LHBs可作为抗病毒治疗效果评价指标的有益补充.
목적 탐토을형간염병독표면대단백(LHBs)재감측항병독료효상적림상의의.방법 근종측46례아덕복위지치료환자60주,분별채용ELISA법、시간분변면역형광분석법화실시정량PCR법검측환자불동시간적LHBs、HBV혈청학표지물화HBV DNA재량,병진행상관성분석.결과 LHBs화HBV DNA하강추세일치,기상관계수r=0.97,단LHBs소퇴만우HBV DNA.치료60주시공유20례환자적HBV DNA<5×102/mL,기중8례LHBs전음;료효반복조14례환자HBVDNA전음후우전양,3례환자LHBs전음;치료무효조12례환자,2례LHBs전음.결론 동태감측LHBs가작위항병독치료효과평개지표적유익보충.
Objective To evaluate hepatitis B virus large surfsce protein(LHBs) in monitoring of antiviral treatment.Methods LHBs.HBV serum markers and HBV DNA loads in 46 patients with adefovir dipivoxil(ADV) therapy were monitored for the whole course(60 weeks).Enzyme linked immunosorbent assay(ELISA),time differentiate immunofluoresence assay and real.time polymerase chain reaction(RT-PCR)were performed to detect LHBs,HBV serum markers and HBV DNA loads,respectively.And correlation analysis was also performed.Results Both LHBs and HBV DNA declined during the ADV treatment.and the correlation coefficient was 0.97.but LHBs declined after HBV DNA.There were 20 patients with HBV DNA<5×102/mL at 60th week.in which 8 were LHBs negative;in 14 recurrent patients,the HBV DNA turned to negative and became positive again,3 with negative LHBs;while in 12 patients resistant to the ADV therapy.2 were LHBs negative.Conclusion Dynamic monitoring of LHBs is useful in the evaluation of antiviral treatment.