中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
11期
743-745
,共3页
免疫组织化学%人骨髓基质干细胞%二膦酸盐类%过氧化物酶体增殖物激活受体γ
免疫組織化學%人骨髓基質榦細胞%二膦痠鹽類%過氧化物酶體增殖物激活受體γ
면역조직화학%인골수기질간세포%이련산염류%과양화물매체증식물격활수체γ
Immunohistochemistry%Human bone mesenchymal stem cells%Diphosphates%proliferator-activated receptor γ
目的 探讨二膦酸盐对糖皮质激素诱导的骨质疏松过氧化物酶体增殖物激活受体γ(PPARγ)表达的影响.方法 ①22例系统性红斑狼疮(SLE)患者,治疗组9例(标准剂量激素+阿仑膦酸钠),对照组13例(标准剂量激素),24周后行骨髓穿刺术,检测免疫组织化学PPARγ平均积分吸光度.②采用原代人骨髓基质干细胞(hMSCs)培养,模拟髓内骨髓基质干细胞分化为脂肪细胞的过程,在相同条件下,分为成脂诱导组、成脂诱导+10-5mol/L二膦酸盐组(高剂量组)、成脂诱导+10-7mol/L二膦酸盐组(中剂量组)、成脂诱导+10-9mol/L二膦酸盐组(低剂量组)干预诱导成脂过程,6~9 d后进行油红0染色、异丙醇萃取油红染料,515 nm测定各组吸光度(A)来反映成脂程度,定量聚合酶链反应(PCR)测定各组PPARγmRNA的表达水平.采用t检验和单因素方差分析法进行数据分析.结果 二膦酸盐治疗组免疫组织化学PPARγ平均积分吸光度较对照组减少(P<0.05);在诱导培养7 d后,成脂诱导组的A与低剂量组差异无统计学意义(P值0.167),高剂量组、中剂量组A与成脂诱导组之间差异有统计学意义(P值分别为0、0.041).定量PCR结果显示:高剂量组、中剂量组PPARγ mRNA的表达低于成脂诱导组(P值分别为0、0.01),低剂量组与成脂诱导组之间差异无统计学意义(P>0.05).结论 有效浓度的二膦酸盐可以抑制PPARγ的表达,从而抑制hMSCs向脂肪细胞分化,抑制糖皮质激素诱导的骨质疏松症.
目的 探討二膦痠鹽對糖皮質激素誘導的骨質疏鬆過氧化物酶體增殖物激活受體γ(PPARγ)錶達的影響.方法 ①22例繫統性紅斑狼瘡(SLE)患者,治療組9例(標準劑量激素+阿崙膦痠鈉),對照組13例(標準劑量激素),24週後行骨髓穿刺術,檢測免疫組織化學PPARγ平均積分吸光度.②採用原代人骨髓基質榦細胞(hMSCs)培養,模擬髓內骨髓基質榦細胞分化為脂肪細胞的過程,在相同條件下,分為成脂誘導組、成脂誘導+10-5mol/L二膦痠鹽組(高劑量組)、成脂誘導+10-7mol/L二膦痠鹽組(中劑量組)、成脂誘導+10-9mol/L二膦痠鹽組(低劑量組)榦預誘導成脂過程,6~9 d後進行油紅0染色、異丙醇萃取油紅染料,515 nm測定各組吸光度(A)來反映成脂程度,定量聚閤酶鏈反應(PCR)測定各組PPARγmRNA的錶達水平.採用t檢驗和單因素方差分析法進行數據分析.結果 二膦痠鹽治療組免疫組織化學PPARγ平均積分吸光度較對照組減少(P<0.05);在誘導培養7 d後,成脂誘導組的A與低劑量組差異無統計學意義(P值0.167),高劑量組、中劑量組A與成脂誘導組之間差異有統計學意義(P值分彆為0、0.041).定量PCR結果顯示:高劑量組、中劑量組PPARγ mRNA的錶達低于成脂誘導組(P值分彆為0、0.01),低劑量組與成脂誘導組之間差異無統計學意義(P>0.05).結論 有效濃度的二膦痠鹽可以抑製PPARγ的錶達,從而抑製hMSCs嚮脂肪細胞分化,抑製糖皮質激素誘導的骨質疏鬆癥.
목적 탐토이련산염대당피질격소유도적골질소송과양화물매체증식물격활수체γ(PPARγ)표체적영향.방법 ①22례계통성홍반랑창(SLE)환자,치료조9례(표준제량격소+아륜련산납),대조조13례(표준제량격소),24주후행골수천자술,검측면역조직화학PPARγ평균적분흡광도.②채용원대인골수기질간세포(hMSCs)배양,모의수내골수기질간세포분화위지방세포적과정,재상동조건하,분위성지유도조、성지유도+10-5mol/L이련산염조(고제량조)、성지유도+10-7mol/L이련산염조(중제량조)、성지유도+10-9mol/L이련산염조(저제량조)간예유도성지과정,6~9 d후진행유홍0염색、이병순췌취유홍염료,515 nm측정각조흡광도(A)래반영성지정도,정량취합매련반응(PCR)측정각조PPARγmRNA적표체수평.채용t검험화단인소방차분석법진행수거분석.결과 이련산염치료조면역조직화학PPARγ평균적분흡광도교대조조감소(P<0.05);재유도배양7 d후,성지유도조적A여저제량조차이무통계학의의(P치0.167),고제량조、중제량조A여성지유도조지간차이유통계학의의(P치분별위0、0.041).정량PCR결과현시:고제량조、중제량조PPARγ mRNA적표체저우성지유도조(P치분별위0、0.01),저제량조여성지유도조지간차이무통계학의의(P>0.05).결론 유효농도적이련산염가이억제PPARγ적표체,종이억제hMSCs향지방세포분화,억제당피질격소유도적골질소송증.
Objective To investigate the effects of bisphosphonate on the proliferator-activated receptor γ(PRARγ)expression of glucocorticoid-induced osteoporosis. Methods ① Twenty-two cases of systemic lupus erythematosus(SLE)patients with glucocorticoids(>0.5 mg·kg-1 ·d-1)were divided into 2 groups: 9 cases in the treatment group in which patients received alendronate 70 mg qw., 13 cases in the control group without anti-osteoporosis treatment. All of the patients had bone marrow puncture after 24 weeks, bone tissues were embedded, sliced, and the value of average optical density of PPARγwas tested with immunohistochemistry. ② Human bone mesenchymal stem cells were cultured in the same conditions and stimulated to adipocytes. During the adipogenic process, the cells were divided into four groups, three of which were experimental groups treated with 10-5 mol/L(high-dose group), 10-7 mol/L(medium-dose group)and 10-9 mol/L(low-dose group)bisphosphonate respectively, while the other was the control group without bisphosphonate in the medium. The adipocytes were identified by oil Red O stain seven days later. The adipocyte rate was measured by light absorption at 515 nm. The expression of PPARγ was measured by quantitative PCR. Comparisons between groups were tested by One-Way ANOVA analysis and t test. Results The average optical density of PPAR-gamma immunohistochemistry in the treatment group was less than the control group(P<0.05). Seven days later, the absorbance between adipogenic induction group and low-dose group was not significant(P=0.167). but the adipogenic absorbance between the high-dose group, middle dose group and the control groups(P values were 0, 0.041). There was significant difference in the quantitative PCR results between the high-dose group, middle dose group and the expression of PPARγ adipogenic induction group(P value was 0, 0.01), but there was no significant differences between the low-dose group and adipogenic induction group(P>0.05). Conclusion Effective concentrations of bisphosphonate can inhibit the expression of PPARγ which in turn lead to the suppression of hMSCs to the adipocytes. The glucocorticoid-induced osteoporosis is also depressed.