中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
7期
429-434
,共6页
阳帆%张仁利%陈思敏%熊鹰%刘涛%黄达娜%武伟华%李玥
暘帆%張仁利%陳思敏%熊鷹%劉濤%黃達娜%武偉華%李玥
양범%장인리%진사민%웅응%류도%황체나%무위화%리모
登革热%登革热病毒%病毒蛋白质类%基因,病毒%序列分析%流行病学,分子
登革熱%登革熱病毒%病毒蛋白質類%基因,病毒%序列分析%流行病學,分子
등혁열%등혁열병독%병독단백질류%기인,병독%서렬분석%류행병학,분자
Dengue%Dengue virus%Viral proteins%Genes,viral%Sequence analysis%Epidemiology,molecular
目的 分析深圳市2010年登革热暴发疫情的病因,从分子水平探讨流行毒株的生物学特征,追踪其地域来源.方法 采用ELISA、胶体金免疫层析法和荧光PCR检测疑似登革热患者血清中的特异性IgM、IgG抗体和病毒核酸,并用C6/36和BHK-21细胞对早期病例血清进行病毒分离,采用反转录-半套式PCR和荧光PCR方法对其进行型别鉴定.同时扩增病毒E基因后进行序列测定,并与不同国家和地区的登革病毒株进行同源性比较和进化树分析.结果 从疑似登革热患者血清中检测到登革病毒IgM、IgG抗体及登革1型病毒核酸.深圳市登革1型病毒分离株SZ1029与登革1型国际标准株HAWAII 45株、我国福建省Fj231/04株及广东省1997、1999年登革1型病毒流行株GD14/97、GD05/99在E基因上的核苷酸同源性分别为94.8%、99.6%、97.7%和98.5%.基因进化树显示,深圳市登革病毒分离株与马来西亚分离株D1/Malaysia/36000/05、新加坡分离株SG(EHI)DEDI42808和Fj231/04株亲缘关系最近,在进化树的同一分支上,与GZ/80、Taiwan87同属基因Ⅰ亚型.所有患者发病前1个月在深圳市某工地居住,无输血史、无外出史.结论 该次疫情的病因为登革1型病毒感染,该毒株有可能来源于东南亚一带,推测深圳可能存在登革1型病毒的疫源地.
目的 分析深圳市2010年登革熱暴髮疫情的病因,從分子水平探討流行毒株的生物學特徵,追蹤其地域來源.方法 採用ELISA、膠體金免疫層析法和熒光PCR檢測疑似登革熱患者血清中的特異性IgM、IgG抗體和病毒覈痠,併用C6/36和BHK-21細胞對早期病例血清進行病毒分離,採用反轉錄-半套式PCR和熒光PCR方法對其進行型彆鑒定.同時擴增病毒E基因後進行序列測定,併與不同國傢和地區的登革病毒株進行同源性比較和進化樹分析.結果 從疑似登革熱患者血清中檢測到登革病毒IgM、IgG抗體及登革1型病毒覈痠.深圳市登革1型病毒分離株SZ1029與登革1型國際標準株HAWAII 45株、我國福建省Fj231/04株及廣東省1997、1999年登革1型病毒流行株GD14/97、GD05/99在E基因上的覈苷痠同源性分彆為94.8%、99.6%、97.7%和98.5%.基因進化樹顯示,深圳市登革病毒分離株與馬來西亞分離株D1/Malaysia/36000/05、新加坡分離株SG(EHI)DEDI42808和Fj231/04株親緣關繫最近,在進化樹的同一分支上,與GZ/80、Taiwan87同屬基因Ⅰ亞型.所有患者髮病前1箇月在深圳市某工地居住,無輸血史、無外齣史.結論 該次疫情的病因為登革1型病毒感染,該毒株有可能來源于東南亞一帶,推測深圳可能存在登革1型病毒的疫源地.
목적 분석심수시2010년등혁열폭발역정적병인,종분자수평탐토류행독주적생물학특정,추종기지역래원.방법 채용ELISA、효체금면역층석법화형광PCR검측의사등혁열환자혈청중적특이성IgM、IgG항체화병독핵산,병용C6/36화BHK-21세포대조기병례혈청진행병독분리,채용반전록-반투식PCR화형광PCR방법대기진행형별감정.동시확증병독E기인후진행서렬측정,병여불동국가화지구적등혁병독주진행동원성비교화진화수분석.결과 종의사등혁열환자혈청중검측도등혁병독IgM、IgG항체급등혁1형병독핵산.심수시등혁1형병독분리주SZ1029여등혁1형국제표준주HAWAII 45주、아국복건성Fj231/04주급광동성1997、1999년등혁1형병독류행주GD14/97、GD05/99재E기인상적핵감산동원성분별위94.8%、99.6%、97.7%화98.5%.기인진화수현시,심수시등혁병독분리주여마래서아분리주D1/Malaysia/36000/05、신가파분리주SG(EHI)DEDI42808화Fj231/04주친연관계최근,재진화수적동일분지상,여GZ/80、Taiwan87동속기인Ⅰ아형.소유환자발병전1개월재심수시모공지거주,무수혈사、무외출사.결론 해차역정적병인위등혁1형병독감염,해독주유가능래원우동남아일대,추측심수가능존재등혁1형병독적역원지.
Objective To determine the pathogen of a local dengue fever outbreak in Shenzhen city in 2010,and to analyze the molecular characteristics of the epidemic dengue virus strain as well as explore the possible origin.Methods The serum samples collected from the suspect dengue fever cases were detected for IgM, IgG by enzyme-linked immunosorbent assay ( ELISA ),immunochromatography and dengue virus nucleic acid by real-time polymerase chain reaction (PCR).Serum samples from patients with early stage dengue fever were used to isolate virus with C6/36 and BHK-21 cell lines.The type of isolated virus strain was determined by RT-semi-nested-PCR and realtime PCR.E gene of isolated virus strain was amplified by RT-PCR and sequenced.Homology and phylogenetic tree of E gene of Shenzhen dengue virus with the strains isolated from other areas were constructed.Results IgM,IgG and RNA of type 1 dengue virus were detected in serum samples from dengue fever suspected patients.Type 1 dengue virus named DEV1-SZ1029 was successfully isolated from the serum sample.The homology of nucleotide sequence of E gene of SZ1029 strain with standard type 1 dengue virus HAWAII 45,Fj231/04,GD14/97 and GD05/99 were 94.8%,99.6%,97.7% and 98.5 %,respectively.The phylogenetic tree indicated that SZ1029 had the greatest similarity with the D1/Malaysia/36000/05 strain,SG(EHI)DED142808 strain and Fj231/04 strain and they lied in the same branch of the phylogenetic tree.The isolated dengue virus type 1 belonged to genetype Ⅰ with GZ/80,Taiwan87,All patients lived in a certain construction site in Shenzhen and had no recent travel history outside the area in one month before infection.Conclusions The virological,serological and molecular features all identify that the local dengue fever outbreak in Shenzhen in 2010 is caused by type 1 dengue virus and SZ1029 strain may be transferred from Southeast Asian region,and there may be a plague focus in Shenzhen.