中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
7期
586-588
,共3页
林庆玲%蔡彦宁%袁艳鹏%左晓虹%陈彪
林慶玲%蔡彥寧%袁豔鵬%左曉虹%陳彪
림경령%채언저%원염붕%좌효홍%진표
Bmal1%Bmal2%昼夜节律%白细胞%帕金森病
Bmal1%Bmal2%晝夜節律%白細胞%帕金森病
Bmal1%Bmal2%주야절률%백세포%파금삼병
Bmall%Bmal2%Circadian rh~hm%Total leukocytes%Parkinson's disease
目的 研究帕金森病患者时钟基因的表达,探讨帕金森病的分子时钟机制.方法 实验对象为17例帕金森病(PD)患者(9例男性,8例女性)和16例正常人对照(9例男性,7例女性),分别在夜间21:00、00:00、06:00和09:00四个时间点取血样,用实时定量RT-PCR检测时钟基因Bmall和Bmal2的mRNA表达水平.结果 PD患者中,Bmall在21:00、00:00、06:00的表达水平与正常对照差异具有显著性:21:00[(22.17 4±4.09),(51.14±8.31),P=0.003];00:00[(30.30 4±5.45),(100.00 4±24.71),P=0.008];06:00[(19.02 ±3.33),(65.61±14.11),P=0.002].Bma12在21:00、00:00的表达水平与正常对照差异有显著性:21:00[(48.09±7.40),(84.96±9.34),P=0.005];00:00[(65.85±7.88),(100.00±11.78),P=0.025].结论 PD患者的周围分子时钟发生了改变.PD患者时钟基因Bmall和Bma12在外周血的相对表达水平明显减低,为疾病监控和研究疾病对药物的反应性提供分子基础.
目的 研究帕金森病患者時鐘基因的錶達,探討帕金森病的分子時鐘機製.方法 實驗對象為17例帕金森病(PD)患者(9例男性,8例女性)和16例正常人對照(9例男性,7例女性),分彆在夜間21:00、00:00、06:00和09:00四箇時間點取血樣,用實時定量RT-PCR檢測時鐘基因Bmall和Bmal2的mRNA錶達水平.結果 PD患者中,Bmall在21:00、00:00、06:00的錶達水平與正常對照差異具有顯著性:21:00[(22.17 4±4.09),(51.14±8.31),P=0.003];00:00[(30.30 4±5.45),(100.00 4±24.71),P=0.008];06:00[(19.02 ±3.33),(65.61±14.11),P=0.002].Bma12在21:00、00:00的錶達水平與正常對照差異有顯著性:21:00[(48.09±7.40),(84.96±9.34),P=0.005];00:00[(65.85±7.88),(100.00±11.78),P=0.025].結論 PD患者的週圍分子時鐘髮生瞭改變.PD患者時鐘基因Bmall和Bma12在外週血的相對錶達水平明顯減低,為疾病鑑控和研究疾病對藥物的反應性提供分子基礎.
목적 연구파금삼병환자시종기인적표체,탐토파금삼병적분자시종궤제.방법 실험대상위17례파금삼병(PD)환자(9례남성,8례녀성)화16례정상인대조(9례남성,7례녀성),분별재야간21:00、00:00、06:00화09:00사개시간점취혈양,용실시정량RT-PCR검측시종기인Bmall화Bmal2적mRNA표체수평.결과 PD환자중,Bmall재21:00、00:00、06:00적표체수평여정상대조차이구유현저성:21:00[(22.17 4±4.09),(51.14±8.31),P=0.003];00:00[(30.30 4±5.45),(100.00 4±24.71),P=0.008];06:00[(19.02 ±3.33),(65.61±14.11),P=0.002].Bma12재21:00、00:00적표체수평여정상대조차이유현저성:21:00[(48.09±7.40),(84.96±9.34),P=0.005];00:00[(65.85±7.88),(100.00±11.78),P=0.025].결론 PD환자적주위분자시종발생료개변.PD환자시종기인Bmall화Bma12재외주혈적상대표체수평명현감저,위질병감공화연구질병대약물적반응성제공분자기출.
Objective To study the expression of clock genes in Parkinson's disease(PD) and also the molecular clock machinery of PD.Methods Seventeen PD patients(nine men,eight women)and sixteen agematched controls(nine men,seven women) were investigated in this study.Bload samples were collected over a 12h span at 21:00,00:00,06:00 and 09:00.Using a real-time PCR assay,the peripheral molecular clock was examined by measuring Bmall and Bmal2 expression in total leukocytes during the dark span(from 21:00 to 09:00)in PD patients and age-matched healthy controls.Results At PD,the relative abundance of Bmall was significantly lower at 21:00,00:00 and 06:00(21:00:(22.17±4.09)vs(51.14±8.31),P=0.003,00:00:(30.30±5.45)vs(100.00±24.71),P=0.008,06:00:(19.02±3.33)vs(65.61±14.11),P=0.002).The relative Bmal2 levels in PD patients were significantly less abundant than controls at 21:00 and 00:00(21:00:(48.09±7.40)vs(84.96±9.34),P=0.005;00:00:((65.85±7.88)vs(100.00±11.78),P=0.025).Conclusion These results suggest that a peripheral molecular clock is altered in PD patients.In addition,the relative abundance of Bmall and Bmal2 was significantly lower in PD patients versus control subjects,which can provide a molecular basis to help monitor disease progression and response to investigational drugs.
