CAJ | 학술논문

背景:维生素D受体基因5'端启动子区外显子2上存在Fok1酶切位点多态性,这一位点的多态性影响维生素D受体氨基酸的结构,与骨密度变化相关.目的:分析老年男性维生素D受体基因Fok1多态性与骨密度的关系.设计:病例-对照,对比观察.单位:解放军总医院老年医学研究所和解放军第二炮兵总医院内分泌科.对象:选择2002-01/06解放军总医院及解放军第二炮兵总医院门诊就诊的26例老年男性骨质疏松患者为骨质疏松组,平均年龄(70±5)岁.腰椎骨密度均低于峰值骨密度2.0～2.5个标准差.选择同期本院健康体检者老年男性66名为对照组,平均年龄(73±4)岁.纳入对象均对检测项目知情同意,且相互无血缘关系,汉族,北京地区居民.方法:采用聚合酶链反应限制性片段长度多态性分析技术确定维生素D受体基因Fok1基因型,分析老年男性维生素D受体基因Fok1基因型的分布情况.主要观察指标:两组老年男性维生素D受体基因Fok1基因型分布情况.结果:老年男性骨质疏松患者26例及健康老年人66名均进入结果分析.维生素D受体基因Fok1多态位点基因型分别为FF,Ff,ff基因型.对照组维生素D受体基因Fok1多态性FF,Ff,ff基因型频率与骨质疏松组(42%,42%,15%;15%,50%,35%)比较,差异明显(x2=12.078,P＜0.01),等位基因F,f频率与骨质疏松组(64%,36%;40%,60%)比较,差异也明显(x2=8.232,P＜0.01).结论:正常老年男性与骨质疏松症老年男性患者的维生素D受体基因5'端启动子区Fok1酶切位点多态性差异明显.
배경:유생소D수체기인5'단계동자구외현자2상존재Fok1매절위점다태성,저일위점적다태성영향유생소D수체안기산적결구,여골밀도변화상관.목적:분석노년남성유생소D수체기인Fok1다태성여골밀도적관계.설계:병례-대조,대비관찰.단위:해방군총의원노년의학연구소화해방군제이포병총의원내분비과.대상:선택2002-01/06해방군총의원급해방군제이포병총의원문진취진적26례노년남성골질소송환자위골질소송조,평균년령(70±5)세.요추골밀도균저우봉치골밀도2.0～2.5개표준차.선택동기본원건강체검자노년남성66명위대조조,평균년령(73±4)세.납입대상균대검측항목지정동의,차상호무혈연관계,한족,북경지구거민.방법:채용취합매련반응한제성편단장도다태성분석기술학정유생소D수체기인Fok1기인형,분석노년남성유생소D수체기인Fok1기인형적분포정황.주요관찰지표:량조노년남성유생소D수체기인Fok1기인형분포정황.결과:노년남성골질소송환자26례급건강노년인66명균진입결과분석.유생소D수체기인Fok1다태위점기인형분별위FF,Ff,ff기인형.대조조유생소D수체기인Fok1다태성FF,Ff,ff기인형빈솔여골질소송조(42%,42%,15%;15%,50%,35%)비교,차이명현(x2=12.078,P＜0.01),등위기인F,f빈솔여골질소송조(64%,36%;40%,60%)비교,차이야명현(x2=8.232,P＜0.01).결론:정상노년남성여골질소송증노년남성환자적유생소D수체기인5'단계동자구Fok1매절위점다태성차이명현.
BACKGROUND: It is found reported that polymorphism of Fok 1 restriction endonuclease cut site on exon 2 of 5' end start codon of 5' end start codon (SC), which affected the structure of VDR amino acids,and was relative related to bone mineral density(BMD).OBJECTIVE: To analyze the association between Vitamin D receptor gene (Fok 1) polymorphisms and osteoporosis in the elderly men.DESIGN: case-controlled trialstudy.SETTING: Institute of Gerontology, Chinese PLA General Hospital and Department of Endocrinology,Second Artillery General Hospital of Chinese PLA.PARTICIPANTS: A total of 26 elderly men with osteoporosis at out-patients clinic of Chinese PLA General Hospital and Department of Endocrinology,Second Artillery General Hospital of Chinese PLA from January 2002 to June 2002 were selected involved as osteoporosis case group,with and the average age of was (70±5) years, and BMD in osteoporosis group was 2.0-2.5 SD lower than 2.0-2.5 SD of the peak of BMD. Totally 66 healthy men with average age of (70±5)years were selected as control group during at the same time. All the subjects signed the informed consent,who were Beijing inhabitants of Han nationality, and there was no blood relationship among them.METHODS:VDR-Fok1 genotypes in both groups were detected with by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),and distributiondistribution of VDR-Fok 1 genotypes were analyzedanalyzed.MAIN OUTCOME MEASURES: distribution Distribution of VDR-Fok1genotypes in both each groups.RESULTS: Totally 66 healthy elderly men and 26 elderly men with osteoporosis entered analysis of results. The frequencies of FF, Ff and ff genotype were found to be 42%, 42% and 15% in control group, and 15%,50%,35% in osteoporosis group, respectively,and there was significantly different between two groups(x2=12.078,P ＜ 0.01).Frequency of allele were significantly different between control group and osteoporosis group (64%,36% vs 40%,60%, x2=8.232,P ＜ 0.01).CONCLUSION: There is a significant difference in the frequency distrinution of VDR gene start codon polymorphism between healthy elderly men and those with osteoporosis.