中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2006年
14期
176-178
,共3页
马太花%尉杰忠%武慧丽%辛静敏%梁丽云%马存根
馬太花%尉傑忠%武慧麗%辛靜敏%樑麗雲%馬存根
마태화%위걸충%무혜려%신정민%량려운%마존근
脑脊髓炎%百日咳菌苗%大鼠
腦脊髓炎%百日咳菌苗%大鼠
뇌척수염%백일해균묘%대서
背景:国外实验室一般选用近交系Lewis大鼠诱导实验性变态反应性脑脊髓炎模型,但国内缺乏Lewis大鼠.因此,本实验选用了国内数量充足、但不十分敏感的Wistar大鼠,在加用百日咳减毒活菌的情况下,成功地在Wistar大鼠中诱导出实验性变态反应性脑脊髓炎动物模型.目的:探讨在不同部位加用减毒百日咳杆菌对实验性变态反应性脑脊髓炎非敏感品系Wistar大鼠模型诱导的影响.设计:随机对照动物实验.单位:山西大同大学脑科学研究所.材料:实验于2003-03/10在山西大同大学脑科学研究所完成.雌性Wistar大鼠58只随机分为3组:①足背实验性变态反应性脑脊髓炎组24只.②腹腔实验性变态反应性脑脊髓炎组24只.③佐剂组10只.方法:除常规免疫动物外,足背实验性变态反应性脑脊髓炎组大鼠于足背皮内或皮下注射减毒百日咳杆菌0.05 mL/只(含5.0×1010个菌体);腹腔实验性变态反应性脑脊髓炎组大鼠于腹腔注射减毒百日咳杆菌0.05 mL/只(含5.0×1010个菌体);佐剂组则以完全弗氏佐剂代替抗原.主要观察指标:①发病时间.②体质量变化.③临床症状.④脑组织病理情况.结果:58只大鼠均进入结果分析.①发病率和发病时间:足背实验性变态反应性脑脊髓炎组发病率为87.5%(21/24),发病时间为免疫后(10.25±1.67)d,而腹腔实验性变态反应性脑脊髓炎组分别为35.7%(9/24)和(14.8±1.79)d,两组比较差异有显著性意义(P＜0.05).②体质量变化和临床症状:足背实验性变态反应性脑脊髓炎组体质量变化为(-16.00±7.30)g,症状评分为3.4±0.7;腹腔实验性变态反应性脑脊髓炎组体质量变化为(-9.14±13.11)g,症状评分为2.4±0.5.③脑组织病理情况:佐剂组大鼠脑脊髓组织没有或有少数单个核细胞浸润.实验性变态反应性脑脊髓炎大鼠炎症病灶侵犯脊髓腰膨大的白质以及灰白质交界处、软脊膜及脊髓实质,大脑皮质及皮髓质交界处甚至深部髓质、脑脊膜和侧脑室周围也被侵犯.病灶也累及小脑、脑干和视交叉,这与观察到的共济障碍、抽搐等表现相一致.苏木精-伊红染色显示病变血管周围有淋巴细胞和单核细胞浸润,呈典型的袖套样改变.足背实验性变态反应性脑脊髓炎和腹腔实验性变态反应性脑脊髓炎发病大鼠均有典型的袖套样改变,但足背实验性变态反应性脑脊髓炎大鼠病灶多.结论:足背皮下注射百日咳毒素诱导实验性变态反应性脑脊髓炎模型病程、病理改变、临床表现都很典型,且发病率高,经济易行,是一种较为理想的实验性变态反应性脑脊髓炎模型诱导方法.
揹景:國外實驗室一般選用近交繫Lewis大鼠誘導實驗性變態反應性腦脊髓炎模型,但國內缺乏Lewis大鼠.因此,本實驗選用瞭國內數量充足、但不十分敏感的Wistar大鼠,在加用百日咳減毒活菌的情況下,成功地在Wistar大鼠中誘導齣實驗性變態反應性腦脊髓炎動物模型.目的:探討在不同部位加用減毒百日咳桿菌對實驗性變態反應性腦脊髓炎非敏感品繫Wistar大鼠模型誘導的影響.設計:隨機對照動物實驗.單位:山西大同大學腦科學研究所.材料:實驗于2003-03/10在山西大同大學腦科學研究所完成.雌性Wistar大鼠58隻隨機分為3組:①足揹實驗性變態反應性腦脊髓炎組24隻.②腹腔實驗性變態反應性腦脊髓炎組24隻.③佐劑組10隻.方法:除常規免疫動物外,足揹實驗性變態反應性腦脊髓炎組大鼠于足揹皮內或皮下註射減毒百日咳桿菌0.05 mL/隻(含5.0×1010箇菌體);腹腔實驗性變態反應性腦脊髓炎組大鼠于腹腔註射減毒百日咳桿菌0.05 mL/隻(含5.0×1010箇菌體);佐劑組則以完全弗氏佐劑代替抗原.主要觀察指標:①髮病時間.②體質量變化.③臨床癥狀.④腦組織病理情況.結果:58隻大鼠均進入結果分析.①髮病率和髮病時間:足揹實驗性變態反應性腦脊髓炎組髮病率為87.5%(21/24),髮病時間為免疫後(10.25±1.67)d,而腹腔實驗性變態反應性腦脊髓炎組分彆為35.7%(9/24)和(14.8±1.79)d,兩組比較差異有顯著性意義(P＜0.05).②體質量變化和臨床癥狀:足揹實驗性變態反應性腦脊髓炎組體質量變化為(-16.00±7.30)g,癥狀評分為3.4±0.7;腹腔實驗性變態反應性腦脊髓炎組體質量變化為(-9.14±13.11)g,癥狀評分為2.4±0.5.③腦組織病理情況:佐劑組大鼠腦脊髓組織沒有或有少數單箇覈細胞浸潤.實驗性變態反應性腦脊髓炎大鼠炎癥病竈侵犯脊髓腰膨大的白質以及灰白質交界處、軟脊膜及脊髓實質,大腦皮質及皮髓質交界處甚至深部髓質、腦脊膜和側腦室週圍也被侵犯.病竈也纍及小腦、腦榦和視交扠,這與觀察到的共濟障礙、抽搐等錶現相一緻.囌木精-伊紅染色顯示病變血管週圍有淋巴細胞和單覈細胞浸潤,呈典型的袖套樣改變.足揹實驗性變態反應性腦脊髓炎和腹腔實驗性變態反應性腦脊髓炎髮病大鼠均有典型的袖套樣改變,但足揹實驗性變態反應性腦脊髓炎大鼠病竈多.結論:足揹皮下註射百日咳毒素誘導實驗性變態反應性腦脊髓炎模型病程、病理改變、臨床錶現都很典型,且髮病率高,經濟易行,是一種較為理想的實驗性變態反應性腦脊髓炎模型誘導方法.
배경:국외실험실일반선용근교계Lewis대서유도실험성변태반응성뇌척수염모형,단국내결핍Lewis대서.인차,본실험선용료국내수량충족、단불십분민감적Wistar대서,재가용백일해감독활균적정황하,성공지재Wistar대서중유도출실험성변태반응성뇌척수염동물모형.목적:탐토재불동부위가용감독백일해간균대실험성변태반응성뇌척수염비민감품계Wistar대서모형유도적영향.설계:수궤대조동물실험.단위:산서대동대학뇌과학연구소.재료:실험우2003-03/10재산서대동대학뇌과학연구소완성.자성Wistar대서58지수궤분위3조:①족배실험성변태반응성뇌척수염조24지.②복강실험성변태반응성뇌척수염조24지.③좌제조10지.방법:제상규면역동물외,족배실험성변태반응성뇌척수염조대서우족배피내혹피하주사감독백일해간균0.05 mL/지(함5.0×1010개균체);복강실험성변태반응성뇌척수염조대서우복강주사감독백일해간균0.05 mL/지(함5.0×1010개균체);좌제조칙이완전불씨좌제대체항원.주요관찰지표:①발병시간.②체질량변화.③림상증상.④뇌조직병리정황.결과:58지대서균진입결과분석.①발병솔화발병시간:족배실험성변태반응성뇌척수염조발병솔위87.5%(21/24),발병시간위면역후(10.25±1.67)d,이복강실험성변태반응성뇌척수염조분별위35.7%(9/24)화(14.8±1.79)d,량조비교차이유현저성의의(P＜0.05).②체질량변화화림상증상:족배실험성변태반응성뇌척수염조체질량변화위(-16.00±7.30)g,증상평분위3.4±0.7;복강실험성변태반응성뇌척수염조체질량변화위(-9.14±13.11)g,증상평분위2.4±0.5.③뇌조직병리정황:좌제조대서뇌척수조직몰유혹유소수단개핵세포침윤.실험성변태반응성뇌척수염대서염증병조침범척수요팽대적백질이급회백질교계처、연척막급척수실질,대뇌피질급피수질교계처심지심부수질、뇌척막화측뇌실주위야피침범.병조야루급소뇌、뇌간화시교차,저여관찰도적공제장애、추휵등표현상일치.소목정-이홍염색현시병변혈관주위유림파세포화단핵세포침윤,정전형적수투양개변.족배실험성변태반응성뇌척수염화복강실험성변태반응성뇌척수염발병대서균유전형적수투양개변,단족배실험성변태반응성뇌척수염대서병조다.결론:족배피하주사백일해독소유도실험성변태반응성뇌척수염모형병정、병리개변、림상표현도흔전형,차발병솔고,경제역행,시일충교위이상적실험성변태반응성뇌척수염모형유도방법.
BACKGROUND: The animals commonly used to induce experimental allergic encephalomyelitis (EAE) in oversea laboratory are rodentia animals such as Lewis rats. But in China we are short of Lewis rats. The un-susceptive animal Wistar rats are inexpensive and plentiful. The adding of pertussis toxin may induce EAE successfully in EAE un-susceptive Wistar rats.OBJECTIVE: To investigate the influence of pertussis toxin injected atdifferent sites in inducing EAE model in un-susceptive Wistar rats.DESIGN: A randomized control animal experiment.SETTING: Institute of Brain Science, Shanxi Datong University.MATERIALS: The study was performed in the Institute of Brain Science of Shanxi Datong University from March to October in 2003. Fifty-eight und adjuvant (CFA) group (n=10).METHODS: Besides routine immunization, each rat in the foot dorsum EAE group and intraperitoneal EAE group was administrated with 005 mL pertussis toxin (containing 5.0×1010 thalli), which were given intraperitoneally and subcutaneously on one hind foot respectively, and the antigen in the CFA group was replaced by CFA.cidence rate and tine of atta ck: In the foot dorsum EAE group, the incidence of EAE was 87.5% (21/24), and the time of attack was at (10.25 ±1.67) days after immunization, which were significantly different from those in the intraperitoneal EAE group [35.7% (9/24), (14.8±l.79) days, P sum EAE group, the change of body mass was (-16.00±7.30) g and the symptomscpre was 3.4±0.7, and those in the intraperitoneal EAE group Therewere no or little infiltration of inflammatory cells in the encephalon and spinal cord of CFA rats. In the EAE rats, there were inflammatory cells infiltrated in the boundary of white matter and gray matter of lumbar intumescence, spinal pia mater, spinal parenehyma, and the boundary of cerebral cortex and medulla, even deep medulla, meninges and around lateral ventricle. There were also mild inflammations in the cerebellum,brainstem and optic chiasma, which were concordant with the observed asynchronism, tic, etc. Hematoxylin and eosin (HE) staining displayed that the infiltrated mononuclear cells assembled in perivascular spaces, which were identified by morphological criteria as lymphocyte and macrophages.Forming typical muff-like changes, the inflammation was less severe in intraperitoneal EAE group than in subcutaneous foot dorsum EAE group.CONCLUSION: The EAE model induced in Wistar rats by Pertussis toxin administered subcutaneously on foot dorsum has the representative course of diseases, pathology change and clinical manifestation and the incidence of diseases is high and the cost is low. So it is a more ideal EAE model inducing method.
