CAJ | 학술논문

目的：探讨血清透明质酸（hyalu ronic acid，HA）浓度与肝脏低温保存-灌注损伤的关系。方法：在成功建立大鼠原位肝移植模型的基础上，将实验动物分为3组：(1)对照(A)组；(2)低温保存 2 h后移植（B）组；（3）低温保存4 h后移植（C）组。分别在术后2、4 h取血清和肝脏标本检测HA及部分肝功能指标，并进行病理观察。结果：移植后血清HA 水平比传统的肝功能指标如乳酸脱氢酶(LDH)、天冬氨酸转氨酶(AST)升高早，下降快。在B 组和C组，血清HA水平明显升高，与A组相比差异显著(P<0.01)。B组和C组可见血管内皮细胞损伤、肝窦和肝小叶结构紊乱。C组移植后4 h标本炎细胞浸润明显。结论：肝脏低温保存导致明显的内皮细胞损伤，再灌注后损伤加重。血清HA水平可反映冷缺血-再灌注损伤的程度
목적：탐토혈청투명질산（hyalu ronic acid，HA）농도여간장저온보존-관주손상적관계。방법：재 성공건립대서원위간이식모형적기출상，장실험동물분위3조：(1)대조(A)조；(2)저온보존 2 h후이식（B）조；（3）저온보존4 h후이식（C）조。분별재술후2、4 h취혈청화간장표 본검측HA급부분간공능지표，병진행병리관찰。결과：이식후혈청HA 수평비전통적간공능지표여유산탈경매(LDH)、천동안산전안매(AST)승고조，하강쾌。재B 조화C조，혈청HA수평명현승고，여A조상비차이현저(P<0.01)。B조화C조가견혈관내피 세포손상、간두화간소협결구문란。C조이식후4 h표본염세포침윤명현。결론：간장저온보존도치명현적내피세포손상，재관주후손상가중。혈청HA수평가반영 랭결혈-재관주손상적정도
Objective: To investigate the relation between ser um hyaluronic acid (HA) concentration and cryopreservation-reperfusion injury. Methods: The animals were randomly assigned to 3 groups: (1 ) group A: the control; (2) group B: liver allografts were stored in lactated R inger's solution (0℃) for 2 h before implantation; (3) group C:liver allografts were stored in lactated Ringer's solution (0℃) for 4 h before implantation. Th e serum sample and liver specimen were taken up at 2 h and 4 h after transplanta tion to detect the concentration of HA, AST and LDH, and to get pathologic obser vation. Results: Serum HA increased earlier and decreased more s hortly than AST and LDH after transplantation in group A. Serum HA increased sig nificantly in group B and C, much higher than that in group A(P<0.01). The i njury of vascular endothelium and the disorder of hepatic sinuses and hepatic lo b ules were observed in group B and C. In the specimen of 4 h in group C, evident infiltration of inflammatory cell was present. Conclusion: Cryopreservation leads to injury of endothelial cell and reperfusion aggravat es this injury. The serum HA concentration indicates the degree of cold ischemia -reperfusion injury.