CAJ | 학술논문

目的利用商陆培养细胞对6β-santonin (1)进行生物转化,以获得多种利于进一步进行结构改造的衍生物.方法将底物1与商陆培养细胞共孵化,采用色谱分离技术得到产物,通过理化性质和光谱数据对产物进行结构鉴定.结果与商陆培养细胞共孵化7 d后,底物1转化为5个产物(2～6),其中3为新化合物.结论利用商陆培养细胞可对6β-santonin进行选择性还原及羟基化反应,可为其进一步化学结构改造提供有价值的中间体.
목적이용상륙배양세포대6β-santonin (1)진행생물전화,이획득다충리우진일보진행결구개조적연생물.방법장저물1여상륙배양세포공부화,채용색보분리기술득도산물,통과이화성질화광보수거대산물진행결구감정.결과여상륙배양세포공부화7 d후,저물1전화위5개산물(2～6),기중3위신화합물.결론이용상륙배양세포가대6β-santonin진행선택성환원급간기화반응,가위기진일보화학결구개조제공유개치적중간체.
Aim To obtain more valuable derivatives for the further structural modification of 6β-santonin (1) via biotransformation by using cell suspension cultures of Phytolacca acinosa. Methods The substrate 1 was incubated with cell suspension cultures of P.acinosa, the products were obtained by chromatography, and identified on the basis of their physical and spectral data (HRMS, 1D NMR, 2D NMR, NOE and IR). Results After incubation with cell suspension cultures of P.acinosa, 1 was converted into five products (2-6). Among them, 3 is a new compound. Conclusion 6β-santonin could be selectively reduced and hydroxylated by the cell suspension cultures of P.acinosa, which would provide valuable intermediates for its further structural modification.