中国药学（英文版）
中國藥學（英文版）
중국약학（영문판）
JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES
2004年
2期
92-96
,共5页
潘云雪%周长新%张水利%郑筱祥%赵昱
潘雲雪%週長新%張水利%鄭篠祥%趙昱
반운설%주장신%장수리%정소상%조욱
毛茛%黄酮苷%芹菜素-4'-O-α-L-鼠李糖苷%芹菜素-8-C-α-L-阿拉伯糖苷%芹菜素-7-O-β-D-葡萄糖苷-4'-O-α-L-鼠李糖苷%细胞毒性
毛茛%黃酮苷%芹菜素-4'-O-α-L-鼠李糖苷%芹菜素-8-C-α-L-阿拉伯糖苷%芹菜素-7-O-β-D-葡萄糖苷-4'-O-α-L-鼠李糖苷%細胞毒性
모간%황동감%근채소-4'-O-α-L-서리당감%근채소-8-C-α-L-아랍백당감%근채소-7-O-β-D-포도당감-4'-O-α-L-서리당감%세포독성
Ranunculus sieboldii Miq.%flavonoid glycosides%apigenin-4'- O-α-L-rhamnopyranoside%apigenin-8- C-α- L-arabinopyranoside%apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside%cytotoxicity
目的研究扬子毛茛的化学成分.方法应用多种色谱方法和色谱材料进行提取、分离和纯化,用各种现代光谱方法并结合文献对分得的化合物进行结构解析.结果从扬子毛茛的95%乙醇提取物中分到十三个化合物,其中五个为黄酮苷类化合物,分别命名为芹菜素-4'-O-α-L-鼠李糖苷(1),芹菜素-7-O-β-D-葡萄糖苷-4'-O-α-L-鼠李糖苷(2),芹菜素-8-C-α-L-阿拉伯糖苷(3),芹菜素-8-C-β-D-半乳糖苷(4),小麦黄素-7-O-β-D-葡萄糖苷(5);其余八个分别为小麦黄素(6),木犀草素(7),东莨菪内酯(8),秦皮乙素(9),滨蒿内酯(10),阿魏酸(11),原儿茶酸(12)和小毛茛内酯(13).此外还对这些化合物的体外抗癌活性进行了初步的测试.结论化合物1-12为首次从该属植物中分到,化合物13为首次从该种植物中分到,其中化合物1为新的天然产物,化合物2和3首次报道了其碳谱数据.生物活性测试结果表明化合物1对BEL-7407及A549细胞的IC50值分别为43及77μg·mL-1,化合物8和10对KB细胞的IC50分别为78和44μg·mL-1,对HL-60细胞的IC50均为85μg·mL-1.化合物7对所测试的四种肿瘤细胞株均显示一定的细胞毒活性,其对KB,BEL-7407,A549及HL-60细胞的IC50分别为51,55,44和10μg·mL-1.
目的研究颺子毛茛的化學成分.方法應用多種色譜方法和色譜材料進行提取、分離和純化,用各種現代光譜方法併結閤文獻對分得的化閤物進行結構解析.結果從颺子毛茛的95%乙醇提取物中分到十三箇化閤物,其中五箇為黃酮苷類化閤物,分彆命名為芹菜素-4'-O-α-L-鼠李糖苷(1),芹菜素-7-O-β-D-葡萄糖苷-4'-O-α-L-鼠李糖苷(2),芹菜素-8-C-α-L-阿拉伯糖苷(3),芹菜素-8-C-β-D-半乳糖苷(4),小麥黃素-7-O-β-D-葡萄糖苷(5);其餘八箇分彆為小麥黃素(6),木犀草素(7),東莨菪內酯(8),秦皮乙素(9),濱蒿內酯(10),阿魏痠(11),原兒茶痠(12)和小毛茛內酯(13).此外還對這些化閤物的體外抗癌活性進行瞭初步的測試.結論化閤物1-12為首次從該屬植物中分到,化閤物13為首次從該種植物中分到,其中化閤物1為新的天然產物,化閤物2和3首次報道瞭其碳譜數據.生物活性測試結果錶明化閤物1對BEL-7407及A549細胞的IC50值分彆為43及77μg·mL-1,化閤物8和10對KB細胞的IC50分彆為78和44μg·mL-1,對HL-60細胞的IC50均為85μg·mL-1.化閤物7對所測試的四種腫瘤細胞株均顯示一定的細胞毒活性,其對KB,BEL-7407,A549及HL-60細胞的IC50分彆為51,55,44和10μg·mL-1.
목적연구양자모간적화학성분.방법응용다충색보방법화색보재료진행제취、분리화순화,용각충현대광보방법병결합문헌대분득적화합물진행결구해석.결과종양자모간적95%을순제취물중분도십삼개화합물,기중오개위황동감류화합물,분별명명위근채소-4'-O-α-L-서리당감(1),근채소-7-O-β-D-포도당감-4'-O-α-L-서리당감(2),근채소-8-C-α-L-아랍백당감(3),근채소-8-C-β-D-반유당감(4),소맥황소-7-O-β-D-포도당감(5);기여팔개분별위소맥황소(6),목서초소(7),동랑탕내지(8),진피을소(9),빈호내지(10),아위산(11),원인다산(12)화소모간내지(13).차외환대저사화합물적체외항암활성진행료초보적측시.결론화합물1-12위수차종해속식물중분도,화합물13위수차종해충식물중분도,기중화합물1위신적천연산물,화합물2화3수차보도료기탄보수거.생물활성측시결과표명화합물1대BEL-7407급A549세포적IC50치분별위43급77μg·mL-1,화합물8화10대KB세포적IC50분별위78화44μg·mL-1,대HL-60세포적IC50균위85μg·mL-1.화합물7대소측시적사충종류세포주균현시일정적세포독활성,기대KB,BEL-7407,A549급HL-60세포적IC50분별위51,55,44화10μg·mL-1.
Aim To investigate the chemical composition of Ranunculus sieboldii Miq.. Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gel filtration on sophadex LH-20 were used to isolate chemical constituents, and their structures were elucidated by extensive spoctroscopic methods (UV, IR, MS, 1H NMR, 13C NMR) including 2D NMR (COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported in literature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1), apigenin- 7- O-β-D-glucopyranosyl-4'- O-α-L-rhamnopyranoside (2), apigenin-8- C-α-L-arabinopyranoside (3), apigenin-8- C-β-D-ga- lactopyranoside ( 4 ), tricin-7- O-β-D-glucopyranoside (5), together with tricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11), protocatechuic acid (12), and tematolide (13) were isolated from the 95% ethanolic extract of its whole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1 - 12 were obtained from this genus and compound 13 from this species for the first time. Furthermore, compound 1 was for the first time isolated from nature while the 13 C NMR data of compounds 2 and 3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407 and A549 cell lines (IC50 43, 77 μg·mL-1 ), 8 and 10 showed inhibitory ac- tivities on KB cell lines (IC50 78, 44 μg· mL- 1 ) and HL-60 cell lines ( IC50 85, 85 μg· mL- 1 ), while 7 exerted moderate cytotoxic activities on KB, BEL-7407, A549 and HL-60 cell lines with their IC50 being 51, 55, 44 and 10 μg·mL-1 , respecfvely.
