南京医科大学学报(英文版)
南京醫科大學學報(英文版)
남경의과대학학보(영문판)
JOURNAL OF NANJING MEDICAL UNIVERSITY
2004年
1期
1-3
,共3页
陈洁%汪红仪%陶金%徐华娥%杨荣%李胜男
陳潔%汪紅儀%陶金%徐華娥%楊榮%李勝男
진길%왕홍의%도금%서화아%양영%리성남
Urocortin%内皮细胞%动脉平滑肌细胞%MTT法%ATP敏感的钾离子通道
Urocortin%內皮細胞%動脈平滑肌細胞%MTT法%ATP敏感的鉀離子通道
Urocortin%내피세포%동맥평활기세포%MTT법%ATP민감적갑리자통도
urocortin%ECV304%vascular smooth muscle cells%MTT assay%ATP-sensitive potassium channels
目的:本实验目的在于研究Urocortin(Ucn)对内皮细胞(一种取自人脐静脉内皮细胞HUVEC ECV304)以及大鼠动脉平滑肌细胞(Vascular smooth muscle cells,VSMC)增值的影响,从而探讨其在血管重构(Vascular Remodeling,YR)中的作用.方法:通过四唑盐比色法研究Ucn对内皮细胞以及鼠平滑肌细胞增值的影响.结果:Ucn(10 -7mol/L)抑制ECV304和VSMC细胞的增值.这种抑制作用不依赖于作用时间也不受ATP敏感的钾离子通道阻滞剂glybenclaimide(Gly,10 μmol/L)的影响.结论:提示Ucn不依赖钾离子通道的作用来控制血管重构.可能对高血压的脑或其他器官的并发症有防治作用,可以作为一种新的血管活性药物.
目的:本實驗目的在于研究Urocortin(Ucn)對內皮細胞(一種取自人臍靜脈內皮細胞HUVEC ECV304)以及大鼠動脈平滑肌細胞(Vascular smooth muscle cells,VSMC)增值的影響,從而探討其在血管重構(Vascular Remodeling,YR)中的作用.方法:通過四唑鹽比色法研究Ucn對內皮細胞以及鼠平滑肌細胞增值的影響.結果:Ucn(10 -7mol/L)抑製ECV304和VSMC細胞的增值.這種抑製作用不依賴于作用時間也不受ATP敏感的鉀離子通道阻滯劑glybenclaimide(Gly,10 μmol/L)的影響.結論:提示Ucn不依賴鉀離子通道的作用來控製血管重構.可能對高血壓的腦或其他器官的併髮癥有防治作用,可以作為一種新的血管活性藥物.
목적:본실험목적재우연구Urocortin(Ucn)대내피세포(일충취자인제정맥내피세포HUVEC ECV304)이급대서동맥평활기세포(Vascular smooth muscle cells,VSMC)증치적영향,종이탐토기재혈관중구(Vascular Remodeling,YR)중적작용.방법:통과사서염비색법연구Ucn대내피세포이급서평활기세포증치적영향.결과:Ucn(10 -7mol/L)억제ECV304화VSMC세포적증치.저충억제작용불의뢰우작용시간야불수ATP민감적갑리자통도조체제glybenclaimide(Gly,10 μmol/L)적영향.결론:제시Ucn불의뢰갑리자통도적작용래공제혈관중구.가능대고혈압적뇌혹기타기관적병발증유방치작용,가이작위일충신적혈관활성약물.
Objective:This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 ceils and VSMC by using a tetrazolium (MTT) assay. Results: Ucn (10-7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively( P< 0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC.Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling(VR).