CAJ | 학술논문

家蚕雌蛾性附腺在化蛾前2到3天开始大量分泌胶状粘性蛋白,其贮存部迅速地膨大,而其Ng突变体的雌蛾性附腺不能正常分泌胶状粘性物质.分别对家蚕(Bombyx mori)的正常及Ng突变体雌蛾性附腺分泌部组织的蛋白质进行提取,并采用双向凝胶电泳和计算机辅助分析方法,对提取的蛋白质混合物进行分离和比较分析,并对主要差异表达的蛋白质用质谱鉴定.实验结果表明,用银染法,平均每张电泳图谱可以分离约700个蛋白质点,其中大部分的蛋白质点分布在pH 4～8范围内,其分子质量主要集中在30～70 ku区域.比较分析发现一些差异表达蛋白,其中No2,3蛋白质点经质谱鉴定为肌动蛋白A3,该蛋白质只在化蛹后期正常雌性附腺组织中特异表达,而Ng突变体中肌动蛋白A3的缺失,暗示了肌动蛋白A3可能与家蚕雌性附腺的胶状粘性物质的胞外分泌有关.
가잠자아성부선재화아전2도3천개시대량분비효상점성단백,기저존부신속지팽대,이기Ng돌변체적자아성부선불능정상분비효상점성물질.분별대가잠(Bombyx mori)적정상급Ng돌변체자아성부선분비부조직적단백질진행제취,병채용쌍향응효전영화계산궤보조분석방법,대제취적단백질혼합물진행분리화비교분석,병대주요차이표체적단백질용질보감정.실험결과표명,용은염법,평균매장전영도보가이분리약700개단백질점,기중대부분적단백질점분포재pH 4～8범위내,기분자질량주요집중재30～70 ku구역.비교분석발현일사차이표체단백,기중No2,3단백질점경질보감정위기동단백A3,해단백질지재화용후기정상자성부선조직중특이표체,이Ng돌변체중기동단백A3적결실,암시료기동단백A3가능여가잠자성부선적효상점성물질적포외분비유관.
The colleterial gland in the silkworm (Bombyx mori) grew gradually until 2 days before emergence, and markedly enlarged due to the accumulation of glue like substances (mainly including 85% water and 11% proteins).However, the Ng mutant female moth only secreted a small amount of glue-like substance and laid loose eggs naturally.High-resolution two-dimensional polyacrylamide gel electrophoresis, followed by computer-assisted analysis was used to screen the proteins pattern between normal and Ng mutant colleterial gland to find quantitative and qualitative difference in proteins expression. Protein spots were resolved in the secretory region of colleterial gland of silkworm and more than 700protein spots were resolved and most of the proteins were distributed in the area from 30 ku to 70 ku and pH 4 ～ 8. Through the comparison and analysis, it was found that 3 proteins were only expressed in the later pupae stage and moth stage.However, these proeins have no expression in the Ng mutant especially spot No. 2 and 3. These differentially expressed proteins were actin identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The results indicated that the actins participate or regulate the exocytosis of colleterial gland and other differential expressed proteins might be having some relations with the glue-like proteins secretion.