CAJ | 학술논문

目的：探讨脑缺血后Fas抗原表达及与细胞凋亡的关系。方法：以鼠局灶脑缺血为动物模型，在缺血后不同时间点（30 min，60 min，24 h，3 d，7 d）分别以免疫组化法和TUNEL法动态检测Fas抗原表达和细胞凋亡情况。结果：缺血后30 min，Fas抗原呈阳性表达，60 min至高峰，24 h开始回落，3 d时已为阴性。TUNEL阳性细胞于缺血后60 min开始出现，3 d达高峰。1周时有回降。结论：脑缺血后有细胞凋亡发生，Fas抗原的大量表达与神经细胞凋亡有密切关系。
목적：탐토뇌결혈후Fas항원표체급여세포조망적관계。방법：이서국조뇌결혈위동물모형，재결혈후불동시간점（30 min，60 min，24 h，3 d，7 d）분별이면역조화법화TUNEL법동태검측Fas항원표체화세포조망정황。결과：결혈후30 min，Fas항원정양성표체，60 min지고봉，24 h개시회락，3 d시이위음성。TUNEL양성세포우결혈후60 min개시출현，3 d체고봉。1주시유회강。결론：뇌결혈후유세포조망발생，Fas항원적대량표체여신경세포조망유밀절관계。
Objective: Our purpose was to study the relationship between the Fas antigen expression and neuron apoptosis after cerebral ischemia. Methods: We detected the Fas antigen expression and neuron apoptosis dynamically in the animal models with cerebral ischemia by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method. Results: Fas antigen was positive after 30 minutes of ischemia and reached peak at 60 minutes. At the 24th hour, it began to decrease, and negative on the 3th day. While the positive cell for TUNEL method appeared after 60 minutes of ischemia, reached peak on 3 day, and decreased on 7 day. Conclusion: Neuron apoptosis after cerebral ischemia is closely related to the over-expression of Fas antigen.