CAJ | 학술논문

分别采用环氧氯丙烷和1,4-二羟基正丁烷双缩水甘油醚活化交联壳聚糖树脂，经偶联抗-乙型肝炎表面抗原(HBsAg)单克隆抗体，制备树脂/抗体免疫吸附剂。含HBsAg的患者阳性血清的吸附实验结果表明，对HBsAg的吸附率可达44%，能使阳性血清转为阴性。通过对不同活化试剂制备的免疫吸附剂的活化过程和吸附性能的研究发现，活化试剂中的"手臂"结构有利于保持偶联抗体的天然构象，使之具有较高的活性。
분별채용배양록병완화1,4-이간기정정완쌍축수감유미활화교련각취당수지，경우련항-을형간염표면항원(HBsAg)단극륭항체，제비수지/항체면역흡부제。함HBsAg적환자양성혈청적흡부실험결과표명，대HBsAg적흡부솔가체44%，능사양성혈청전위음성。통과대불동활화시제제비적면역흡부제적활화과정화흡부성능적연구발현，활화시제중적"수비"결구유리우보지우련항체적천연구상，사지구유교고적활성。
The crosslinked chitosan resins were activated by epichlorohydrin and 1,4-butanediol diglycidyl ether, respectively. The effect factors of activation were studied in detail and the reaction conditions were optimized. Then the anti-HBsAg monoclonal antibodies were bound to the activated carrier to obtain the immunoadsorbent. The adsorption properties of the immunoadsorbent were studied in serum containing HBsAg and the adsorption efficiency reached 44%. With the further studies on the activation and the adsorption, a conclusion was draw that the activation reagent with longer spacer was favor to keep the normal configuration of the antibodies bound to the adsorbent carriers so that the immunoadsorbent was provided with higher bioactivity to react with HBsAg.