CAJ | 학술논문

为获得具有生物学活性的hFKBP52，来筛选新型的促神经再生药物.采用半巢式、桥联PCR及亲和层析方法，从人胎脑cDNA文库中成功扩增出hFKBP52基因，在pET28a（+）中实现了高效、可溶性的融合表达，表达量约30%.重组的蛋白质经亲和纯化至电泳纯，纯化后的 hFKBP52显示出肽基脯氨基顺反异构酶活性.表明原核表达的hFKBP52具有类似于其天然蛋白质的生物学活性.
위획득구유생물학활성적hFKBP52，래사선신형적촉신경재생약물.채용반소식、교 련PCR급친화층석방법，종인태뇌cDNA문고중성공확증출hFKBP52기인，재pET28a（+）중실 현료고효、가용성적융합표체，표체량약30%.중조적단백질경친화순화지전영순，순화후적 hFKBP52현시출태기포안기순반이구매활성.표명원핵표체적hFKBP52구유유사우기천연단백 질적생물학활성.
To obtain active hFKBP52 protein for screening novel neu rotrophic drugs. Semi-nested and overlap PCR and affinity chromatography were u sed. hFKBP52 gene was cloned successfully from human fetal brain cDNA library, a nd then highly expressed (about 30%) as fusion protein in pET28a(+) vector syste m. The recombinant protein was purified as one band on SDS-PAGE. The purified h FKBP52 showed peptidyl-prolyl cis-trans isomerase (PPIase) activity, simil ar to the wild type.