CAJ | 학술논문

研究主要探讨P物质(SP)对GABA-激活电流的调制。实验在培养的新生大鼠海马大锥体细胞上进行, 应用全细胞膜片箝技术记录GABA激活的内向电流。在被检的大锥体细胞中, 有72%(66/92)的神经元对GABA和SP同时敏感。预加SP后, GABA激活电流明显地被抑制, 此抑制作用是呈剂量依赖性的, 在预加10－8、10－7、10－6、10－5 mol/L SP后, GABA的激活电流分别降低18%、24.8%、25.9%和28%。用SP的拮抗剂spantide能阻断此种抑制作用, 在电极中灌注H7 (PKC抑制剂)能取消此抑制作用。上述结果提示: SP对GABA激活电流的抑制作用是SP作用于SP受体, 通过胞内第二信使, 使GABAA受体通道复合体胞内磷酸化所致。
연구주요탐토P물질(SP)대GABA-격활전류적조제。실험재배양적신생대서해마대추체세포상진행, 응용전세포막편겸기술기록GABA격활적내향전류。재피검적대추체세포중, 유72%(66/92)적신경원대GABA화SP동시민감。예가SP후, GABA격활전류명현지피억제, 차억제작용시정제량의뢰성적, 재예가10－8、10－7、10－6、10－5 mol/L SP후, GABA적격활전류분별강저18%、24.8%、25.9%화28%。용SP적길항제spantide능조단차충억제작용, 재전겁중관주H7 (PKC억제제)능취소차억제작용。상술결과제시: SP대GABA격활전류적억제작용시SP작용우SP수체, 통과포내제이신사, 사GABAA수체통도복합체포내린산화소치。
The purpose of the present study was to explore whether substance P (SP) modulates the response mediated by GABAA receptors. Experiments were carried out on cultured hippocampal pyramidal neurons of rats. GABA-activated inward currents were recorded using the whole-cell-patch-clamp techique. The majority of the neurons examined (66/92, 72%) were sensitive to both GABA and SP. When the neurons were treated with SP prior to application of GABA, the GABA-activated current (IGABA) was inhibited markedly, which was concentration-dependent and could be blocked by spantide, an NK1 receptor antagonist. With 10－8, 10－7, 10－6 and 10－5 mol/L SP, IGABA was inhibited by 18%, 24.8%, 25.9% and 28% respectively. Intracellular application of H7, a potent inhibitor of PKC, abolished inhibition of IGABA by SP, suggesting that the inhibition of IGABA by SP may be a result of intracellular phosphorylation of the GABAA receptor.