华东师范大学学报(自然科学版)
華東師範大學學報(自然科學版)
화동사범대학학보(자연과학판)
2001年
2期
78-83
,共6页
绿草履虫%共生小球藻%同工酶
綠草履蟲%共生小毬藻%同工酶
록초리충%공생소구조%동공매
作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。除了对乳酸脱氢酶未检出酶蛋白条带外，其它三种酶的电泳图谱都显示暗培养草履虫的同工酶组成条带多、含量高。作者由所得结果推测小球藻共生体可能改变了宿主细胞基因的表达。
作者應用聚丙烯酰胺凝膠電泳技術分析比較瞭光培養綠草履蟲和暗培養綠草履蟲中酯酶、痠性燐痠酶、蘋果痠脫氫酶和乳痠脫氫酶四種同工酶的組分及含量的變化。除瞭對乳痠脫氫酶未檢齣酶蛋白條帶外，其它三種酶的電泳圖譜都顯示暗培養草履蟲的同工酶組成條帶多、含量高。作者由所得結果推測小毬藻共生體可能改變瞭宿主細胞基因的錶達。
작자응용취병희선알응효전영기술분석비교료광배양록초리충화암배양록초리충중지매、산성린산매、평과산탈경매화유산탈경매사충동공매적조분급함량적변화。제료대유산탈경매미검출매단백조대외，기타삼충매적전영도보도현시암배양초리충적동공매조성조대다、함량고。작자유소득결과추측소구조공생체가능개변료숙주세포기인적표체。
PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase, acid phosphatase, malate dehydrogenase (MDH) and lactate dehydrogenase(LDH), of Paramecium bursaria which cultured in light and dark . Except LDH was not checked up band, the electrophoresis results of other three isoenzymes indicated that the isoenzymes of dark cultivated P. bursaria had more bands and higher content .From these results it can be concluded that the symbiotic Chlorella probably effect the gene expression of the host cell.
