CAJ | 학술논문

采用体外细胞培养的方法，观察复合真菌多糖诱导人肝癌细胞Bel-7402凋亡的影响，利用双重免疫标记方法分析肿瘤抑制基因P53在Bel-7402凋亡中的表达。结果表明：不同浓度的复合真菌多糖均能明显诱导Bel-7402细胞凋亡，并随浓度增加而渐次出现不同时相的细胞凋亡特征性染色质凝聚；能明显降低G1、S期，且有显著的剂量－效应关系，凋亡细胞百分率亦随浓度的增加而逐渐升高；在高浓度组出现典型的DNA阶梯状电泳条带。证明复合真菌多糖抗肿瘤作用机理与诱导肿瘤细胞的凋亡密切相关，其诱导肿瘤细胞凋亡是P53非依赖性。
채용체외세포배양적방법，관찰복합진균다당유도인간암세포Bel-7402조망적영향，이용쌍중면역표기방법분석종류억제기인P53재Bel-7402조망중적표체。결과표명：불동농도적복합진균다당균능명현유도Bel-7402세포조망，병수농도증가이점차출현불동시상적세포조망특정성염색질응취；능명현강저G1、S기，차유현저적제량－효응관계，조망세포백분솔역수농도적증가이축점승고；재고농도조출현전형적DNA계제상전영조대。증명복합진균다당항종류작용궤리여유도종류세포적조망밀절상관，기유도종류세포조망시P53비의뢰성。
To observe the influence of fungus polysaccharides compounds(FPC) in inducing human liver cancer cell Bel-7402 apoptosis in cell cultivating in vitro, the authors analyzed tumor inhibitive gene P53 expression in Bel-7402 apoptosis by applying double immuno-marker. The result showed that the multilevel of FPC could all apparently induce Bel-7402 apoptosis. With the enhancement of FPC concentration, the authors observed chromatin condensation in some phases companying with the characteristic apoptosis. In the meantime, it could also greatly reduce the G1 and S, with obviously dose-response relationship. The percentage of cell apoptosis increased with the enhancing of concentration. In the high-level group the authors found typical DNA ladder eletrophoresis stripe. The result showed that the mechanism of the FPC antineoplastic effect had an intimate relation with its induction to apoptosis and that the result of FPC inducing tumor cell apoptosis had the character of P53 independence.