CAJ | 학술논문

目的探讨Hart改良的弹力纤维染色法在判断胃癌脉管侵袭中的应用价值。方法对66例石蜡包埋胃癌组织分别进行HE染色，Hart改良的弹力纤维染色，光镜下观察66例胃癌的组织学分类、淋巴管和脉管侵入情况。结果 66例胃癌中有血管侵入者50例(74%)，有粘膜下淋巴管侵入者60例(91％)，在癌组织侵袭的淋巴管周围，血管内有癌细胞侵入者47例(78％)。结论 Hart改良的弹力纤维染色法可使一些较细的弹力纤维着色，效果强于其它弹力纤维染色法，特异性与免疫组化相比差别不大，易操作且经济，应当考虑将其作为肿瘤诊断中的一个常规染色法加以应用。
목적 탐토Hart개량적탄력섬유염색법재판단위암맥관침습중적응용개치。방법 대66례석사포매위암조직분별진행HE염색，Hart개량적탄력섬유염색，광경하관찰66례위암적조직학분류、림파관화맥관침입정황。결과 66례위암중유혈관침입자50례(74%)，유점막하림파관침입자60례(91％)，재암조직침습적림파관주위，혈관내유암세포침입자47례(78％)。결론 Hart개량적탄력섬유염색법가사일사교세적탄력섬유착색，효과강우기타탄력섬유염색법，특이성여면역조화상비차별불대，역조작차경제，응당고필장기작위종류진단중적일개상규염색법가이응용。
Objective To study the practical value of Hart improved elasticfiberstain(HIEFS) in determination of vessel invasion in gastric carcinoma. Methods 66 cases of gastric carcinoma tissues were stained with Hematoxylin-eosin staining and HIEFS. Histological classification, lymphatics and vessel invasion were observed by light microscope.Results Among 66 cases of gastric carcinoma tissues, vessel invasion were observed in 50(74%) cases, lymphatics invasion were found in 60 (91%) cases, in which 47 (78%) cases were accompanied with vessel invasion.Conclusion We can get a better result by HIEFS than other elastic fiber stain methods, for it can dye those tiny elastic fiber. No significant difference between HIEFS and IHC. HIEFS is not only easy to operate, but also economic. It should be considered to make it a routine stain method in the work of tumor diagnosis.