CAJ | 학술논문

目的建立与临床冠心病极为相似的慢性多支冠状动脉狭窄大动物模型。方法成年犬19只，在左前降支（LAD）和左回旋支（LCX）近端安放缩窄器，二维超声评价室壁增厚率（%WT），放射性微球定量分析心肌血流量（MBF）。结果术后第7天26个（52%）心肌节段表现为局部室壁运动异常（RWMA），LAD和LCX供血区的平均%WT低于术前（P<0.05）；但LAD和LCX供血区的MBF在术后第21天才降低（P<0.05）。术后第42天有36个（72%）心肌节段表现为RWMA，LAD和LCX供血区的平均%WT亦均低于术前（P<0.05），但仅LCX供血区的MBF保持低下（P<0.05）。结论慢性多支冠状动脉狭窄犬模型可产生明显的节段性左室收缩功能低下，适宜于慢性可逆性心肌收缩功能障碍的研究。
목적건립여림상관심병겁위상사적만성다지관상동맥협착대동물모형。방법성년견19지，재좌전강지（LAD）화좌회선지（LCX）근단안방축착기，이유초성평개실벽증후솔（%WT），방사성미구정량분석심기혈류량（MBF）。결과술후제7천26개（52%）심기절단표현위국부실벽운동이상（RWMA），LAD화LCX공혈구적평균%WT저우술전（P<0.05）；단LAD화LCX공혈구적MBF재술후제21천재강저（P<0.05）。술후제42천유36개（72%）심기절단표현위RWMA，LAD화LCX공혈구적평균%WT역균저우술전（P<0.05），단부LCX공혈구적MBF보지저하（P<0.05）。결론만성다지관상동맥협착견모형가산생명현적절단성좌실수축공능저하，괄의우만성가역성심기수축공능장애적연구。
Objective To establish a large animal model of chronicmulticoronary stenosis or occlusion that mimics human condition of coronary artery disease(CAD). Methods Nineteen adult dogs were used to establish chronic multicoronary stenosis or/and occlusion by installation of Ameroid constrictors at the proximal portion of the left anterior descending (LAD) and the left circumflex coronary arteries(LCX). The wall thickening percentage(%WT)was measured with 2D-echocardiography (2DE) and myocardial blood flow (MBF) with radiolabeled microspheres. Baseline 2DE was performed immediately prior to surgery. At postoperative days 2, 7, 14, 21, 28 and 42,2DE was performed and radiolabled microspheres administered. Results Thirteen dogs completed the whole experiment. At day 7, regional wall motion abnormality (RWMA) was presented in 26 myocardial sections . The average %WT significantly decreased in both LAD and LCX at day 7, while the decrease in MBF was not observed until day 21. At day 42, RWMA was seen in 36 out of 50 sections. The average %WT in both LAD and LCX beds was still lower than the baseline. MBF in the LCX bed decreased further, which returned to nearly the baseline levels. Conclusion This canine model of chronic mutilcoronary stenosis simulating the human condition of CAD produces significant RWMA and is appropriate for the study of chronic reversible myocardial contractile dysfunction.