CAJ | 학술논문

目的探讨高温、噪声环境中飞行对飞行员红细胞膜ATP酶活性的影响.方法轰六飞行员24名为实验组,普通地面人员21名为对照组.作多靶场轰炸飞行,舱内温度45～53℃,噪声97～122dB(A).飞行时间3h,于飞行前(6:00a.m.)、飞行后即刻(12:00a.m.)、8h(8:00p.m.)采血.对照组采血日行室内作业(27℃).用比色法测定红细胞膜Na+-K+ATP酶、Ca2+-Mg2+ATP酶活性.结果实验组和对照组的Na+-K+ATP酶及Ca2+-Mg2+ATP酶日间(6:00a.m.,12:00a.m.,8:00p. m.)变化曲线基本一致并均在正常范围内;实验组清晨红细胞膜Na+-K+ATP酶活性明显高于对照组;实验组飞行后即刻红细胞膜Na+-K+ATP酶活性明显高于飞行后8h,飞行后即刻及8hCa2+-Mg2+ATP酶活性明显高于同一时间对照组.结论长时间高温、噪声刺激可引起ATP的过度消耗,严重时造成红细胞内Ca2+超载,红细胞膜僵硬,甚至红细胞凋亡.
목적탐토고온、조성배경중비행대비행원홍세포막ATP매활성적영향.방법굉륙비행원24명위실험조,보통지면인원21명위대조조.작다파장굉작비행,창내온도45～53℃,조성97～122dB(A).비행시간3h,우비행전(6:00a.m.)、비행후즉각(12:00a.m.)、8h(8:00p.m.)채혈.대조조채혈일행실내작업(27℃).용비색법측정홍세포막Na+-K+ATP매、Ca2+-Mg2+ATP매활성.결과실험조화대조조적Na+-K+ATP매급Ca2+-Mg2+ATP매일간(6:00a.m.,12:00a.m.,8:00p. m.)변화곡선기본일치병균재정상범위내;실험조청신홍세포막Na+-K+ATP매활성명현고우대조조;실험조비행후즉각홍세포막Na+-K+ATP매활성명현고우비행후8h,비행후즉각급8hCa2+-Mg2+ATP매활성명현고우동일시간대조조.결론장시간고온、조성자격가인기ATP적과도소모,엄중시조성홍세포내Ca2+초재,홍세포막강경,심지홍세포조망.
Objective To determine the effect of heat and noise on erythrocyte memb rane ATPase activities in pilots during flying. Method Twenty- four pilots per forming bombing for 3h (45～53℃, 122～97dB in the cabin) served as the su bjects . 21 ground personnel served as control (27℃ in the room). Blood samples were t aken from both groups before flying (6:00 a.m), and immediately (12:00 a.m.) and 8h (8:00 p.m.) after flying. Na+-K+ATPase, and Ca2+-Mg2+ATPase activities in erythrocyte membran e were determined with colorimetry. Result The Na+-K+ATPase activity in eryth rocyte membrane at 6:00 a.m.in pilots was higher than that in control group at the s ame time (P<0.01). The Ca2+-Mg2+ATPase activities in erythrocyte membrane at 12:00 a .m. and 8:00 p.m. in pilots were significantly higher, compared with those in co ntrol group at the same time (P<0.01). Conclusion The ATPase values obtained in our study were all within normal range, and the daytim e variation of both groups are the same. Exposure of human body to heat and noi se for long time may be harmful, the higher ATPase activity is, the more catabol ism of ATP will be. ATP exhaustion will lead to Ca2+ overload in erythrocy te thus stiffen the red cell membrane.