CAJ | 학술논문

利用全细胞膜片钳技术在急性分离的胃窦平滑肌细胞上记录离子电流的方法, 探讨外源性不饱和脂肪酸是否参与低渗牵张加强毒蕈碱电流的过程.在豚鼠胃窦平滑肌细胞上膜电位被钳制在-20.0 mV等渗状态时, 50 μmol/L 卡巴胆碱(carbachol, CCh)引起的毒蕈碱电流(ICCh)作为对照, 发现低渗牵张可以使ICCh明显增加到对照的226.0±21.0%. 当用含5 μmol花生四烯酸(arachid acid, AA)、亚麻酸(linoleic acid, LA)或亚油酸(oleic acid, OA)细胞外液灌流时, ICCh 分别被抑制在对照的3.8±0.6%、 35.2±0.8%和66.6±0.6%.在这种情况下, 低渗牵张刺激可以使ICCh分别增加到106.0±2.5%、 173.2±6.8%和222.1±11.0%.5 μmol/L AA抑制低渗牵张增加的毒蕈碱电流51.2±3.8%, 而在等渗状态下抑制ICCh 为96.2±1.6%.上述结果提示, 不饱和脂肪酸中双键数目越多, 抑制效应越强; 但不饱和脂肪酸不参与低渗刺激加强毒蕈碱电流的过程.
이용전세포막편겸기술재급성분리적위두평활기세포상기록리자전류적방법, 탐토외원성불포화지방산시부삼여저삼견장가강독심감전류적과정.재돈서위두평활기세포상막전위피겸제재-20.0 mV등삼상태시, 50 μmol/L 잡파담감(carbachol, CCh)인기적독심감전류(ICCh)작위대조, 발현저삼견장가이사ICCh명현증가도대조적226.0±21.0%. 당용함5 μmol화생사희산(arachid acid, AA)、아마산(linoleic acid, LA)혹아유산(oleic acid, OA)세포외액관류시, ICCh 분별피억제재대조적3.8±0.6%、 35.2±0.8%화66.6±0.6%.재저충정황하, 저삼견장자격가이사ICCh분별증가도106.0±2.5%、 173.2±6.8%화222.1±11.0%.5 μmol/L AA억제저삼견장증가적독심감전류51.2±3.8%, 이재등삼상태하억제ICCh 위96.2±1.6%.상술결과제시, 불포화지방산중쌍건수목월다, 억제효응월강; 단불포화지방산불삼여저삼자격가강독심감전류적과정.
To investigate the function of exogenous unsaturated fatty acids in hyposmotic membrane stretch enhancement of muscarinic current (ICCh) in antral circular smooth muscle cells of guinea pig, we recorded the membrane current with the conventional whole cell patch-clamp technique. ICCh elicited by 50 μmol/L carbachol (CCh) at the holding potential of -20 mV under isosmotic condition was taken as control. Hyposmotic membrane stretch increased ICCh to 226.0±21.0%. When the cells were pretreated with 5 μmol/L arachidonic acid (AA), linoleic acid (LA) or oleic acid (OA), ICCh was inhibited to 3.8±0.6%, 35.2±0.8% and 66.6±0.6% respectively. Hyposmotic membrane stretch increased ICCh to 106.0±2.5%, 173.2±6.8% and 222.1±11.0% of the control respectively. Five μmol/L AA inhibited hyposmotic membrane stretch-enhanced ICCh by 51.2±3.8%, while the control ICCh under isosmotic condition was inhibited by 96.2±1.6%. The results suggest that unsaturated fatty acids inhibited ICCh and the inhibitory effect is more significant when the unsaturation degree is increased. However, the unsaturated fatty acids are not involved in the increase of ICCh induced by hyposmotic membrane stretch.