水生生物学报
水生生物學報
수생생물학보
ACTA HYDROBIOLOGICA SINICA
2002年
5期
501-504
,共4页
细胞松弛素B%嗜热四膜虫%皮层细胞骨架蛋白
細胞鬆弛素B%嗜熱四膜蟲%皮層細胞骨架蛋白
세포송이소B%기열사막충%피층세포골가단백
Cytochalain B%Tetrahymena thermophila%Conjugation cortical cytoskeletal protein
测定了细胞松弛素B对嗜热四膜虫BF1株和BF5在生殖接合期的皮层骨架蛋白,尤其是在接合子接合膜形成和原核交换阶段影响甚大.作者发现CB处理后 ,与对照组相比较,皮层骨架蛋白146KD消失,27KD、43KD、47KD和174KD含量下降,32KD、 41KD、51KD和54KD保持不变.结果显示,松弛素B对微纤毛蛋白27KD、43KD、47KD、146KD和 174KD有影响.
測定瞭細胞鬆弛素B對嗜熱四膜蟲BF1株和BF5在生殖接閤期的皮層骨架蛋白,尤其是在接閤子接閤膜形成和原覈交換階段影響甚大.作者髮現CB處理後 ,與對照組相比較,皮層骨架蛋白146KD消失,27KD、43KD、47KD和174KD含量下降,32KD、 41KD、51KD和54KD保持不變.結果顯示,鬆弛素B對微纖毛蛋白27KD、43KD、47KD、146KD和 174KD有影響.
측정료세포송이소B대기열사막충BF1주화BF5재생식접합기적피층골가단백,우기시재접합자접합막형성화원핵교환계단영향심대.작자발현CB처리후 ,여대조조상비교,피층골가단백146KD소실,27KD、43KD、47KD화174KD함량하강,32KD、 41KD、51KD화54KD보지불변.결과현시,송이소B대미섬모단백27KD、43KD、47KD、146KD화 174KD유영향.
The effect of cytochalasin B on cortical cytoskelet al proteins of the complementary mating types of Tetrahymena thermophila BF 1 and BF5 during their conjugation was carried out, especially during the in itial stage of fusion membrane formation stage and the stage of pronuclei excha n ge. We found that after the treatment of CB, 146KD cortical cytoskeletal protein disappeared, while it existed in the control group, and the percent of 27KD、43 KD、47KD and 174KD declined, but the percent of 32KD、41KD、51KD and 54KD kept a lmost unchanged. Thus we concluded that 27KD、43KD、47KD、146KD and 174KD may be the microfilament proteins.
