CAJ | 학술논문

从烟台地区采集13份大樱桃根癌病样本,分离纯化出26个菌株,致病性测定表明23个菌株在指示寄主番茄上具有致病性;进一步经生理生化反应、碳源利用、选择性培养基等试验鉴定出16个根癌病菌菌株为Agrobacterium tumefaciens生物型2,占69.57%,为优势种群,其余7个菌株为生物型1.对大樱桃根癌病菌分类地位的确定为今后病原菌的系统研究和病害防治提供了理论基础.
종연태지구채집13빈대앵도근암병양본,분리순화출26개균주,치병성측정표명23개균주재지시기주번가상구유치병성;진일보경생리생화반응、탄원이용、선택성배양기등시험감정출16개근암병균균주위Agrobacterium tumefaciens생물형2,점69.57%,위우세충군,기여7개균주위생물형1.대대앵도근암병균분류지위적학정위금후병원균적계통연구화병해방치제공료이론기출.
26 strains was obtained from 13 samples of crown gall on sweet cherry in Yantai. 23 strains could form crown gall tomato.These strains were identified further based on reactions to standard biochemical and physiological tests,by carbon utilization and by selective media for identification. Among 23 strain,16 strains were Agrobacterium tumefaciens biotype 2,which made 69.57% of total amount. The other 7 strains were A. tumefaciens biotype 1. The result indicated that the dominant biotype of A. tumefaciens was biotype 2 in Yantai. The establishment of A. tumefaciens causing crown gall disease of sweet cherry laid the foundations for systematic study on pathogenic A. tumefaciens and disease control.