CAJ | 학술논문

在真核生物和原核生物的生物膜上都存在由同种受体蛋白相互连接在一起形成的紧密二维排列.最近的模型计算表明这种排列方式可能是一种新型信号转导机制的结构基础,相邻受体可通过功能上的耦联优化信号处理性能.Ryanodine受体(ryanodine receptor,RyR)/钙释放通道通常在肌肉的肌浆网膜上形成二维晶格排列,该蛋白成为研究受体二维排列及其生理功能的一个很好的模型.本文综述了近几年在RyR相互作用及其二维排列工作模式和生理功能研究方面的进展,着重介绍了我们实验室利用新方法对RyR相互作用及其调控进行的研究工作.我们研究中发现了RyR功能状态对其相互作用的调控,本文对据此提出的RyR二维排列的"动态耦联模型"及其可能的生理功能进行了详细讨论.
재진핵생물화원핵생물적생물막상도존재유동충수체단백상호련접재일기형성적긴밀이유배렬.최근적모형계산표명저충배렬방식가능시일충신형신호전도궤제적결구기출,상린수체가통과공능상적우련우화신호처이성능.Ryanodine수체(ryanodine receptor,RyR)/개석방통도통상재기육적기장망막상형성이유정격배렬,해단백성위연구수체이유배렬급기생리공능적일개흔호적모형.본문종술료근궤년재RyR상호작용급기이유배렬공작모식화생리공능연구방면적진전,착중개소료아문실험실이용신방법대RyR상호작용급기조공진행적연구공작.아문연구중발현료RyR공능상태대기상호작용적조공,본문대거차제출적RyR이유배렬적"동태우련모형"급기가능적생리공능진행료상세토론.
Receptor proteins in both eukaryotic and prokaryotic cells often form regular lattice or array in the membrane. Recent theoretical analyses indicate that such arrays may provide a novel mechanism for receptor signaling regulation in cells. The functional coupling between neighboring receptors could improve the signaling performance. The ryanodine receptors (RyR)/calcium release channels usually form 2-D regular lattice in the endoplasmic/sarcoplasmic reticulum membranes. Thus, RyR is a potentially good model to study the function of receptor 2-D array. In this article, we briefly review recent progresses in this research field, including RyR-RyR interaction, RyR array's function and working mechanisms. The investigations performed by new methods in our laboratory are summarized. We demonstrate that the RyR-RyR interaction is modulated by the functional states of RyRs. Accordingly, the mechanism of "dynamic coupling" of RyR array is proposed. Its possible role in RyR-mediated Ca2+ release is discussed.