CAJ | 학술논문

为了研究番茄幼苗在缺磷胁迫下根系形态发育与生长素、生长素信号转导途径中的转录因子NAC1,以及调控NAC1表达的上游miR164之间的关系.试验以5和500 μmol/L磷浓度作为缺磷胁迫和对照,检测了外源生长素NAA(1-naphthalene acetic acid)及生长素抑制剂NPA(N-1-naphthylphthalamic acid)对侧根形成的影响;同时采用RT-PCR检测了NAC1和miR164在缺磷胁迫下的时序表达.结果表明,缺磷胁迫下侧根大量形成与生长素及其运输密切相关,在侧根迅速形成的24 h内,NAC1的表达在缺磷胁迫下增强;而其上游的miR164表达降低,从而揭示了缺磷胁迫下侧根形成与miR164调节NAC1表达之间的关系.
위료연구번가유묘재결린협박하근계형태발육여생장소、생장소신호전도도경중적전록인자NAC1,이급조공NAC1표체적상유miR164지간적관계.시험이5화500 μmol/L린농도작위결린협박화대조,검측료외원생장소NAA(1-naphthalene acetic acid)급생장소억제제NPA(N-1-naphthylphthalamic acid)대측근형성적영향;동시채용RT-PCR검측료NAC1화miR164재결린협박하적시서표체.결과표명,결린협박하측근대량형성여생장소급기운수밀절상관,재측근신속형성적24 h내,NAC1적표체재결린협박하증강;이기상유적miR164표체강저,종이게시료결린협박하측근형성여miR164조절NAC1표체지간적관계.
his study investigated the relationship between the development of tomato lateral roots and auxin, NAC1 (transcription factor involved in the auxin signal transduction) and miR164 expression under P deficiency. Effects of exogenous auxin NAA (1-naphthalene acetic acid) and its transport inhibitor NPA (N-1-naphthylphthalamic acid) on the lateral root development were analyzed under P deficiency (5 μmol/L Pi) versus the control (500 μmol/L Pi). The time course of the expression pattern of miR164 and its target gene, NAC1, were analyzed by RT-PCR. The results showed that the development of lateral roots was promoted by NAA and blocked by NPA. During 12 h of lateral root initiation, expression of NAC1 was strongly up-regulated, while miR164 was down-regulated in P-deficient plants. Our data suggest that miR164, together with its target (NAC1), is involved in the meditation of P-deficiency-induced lateral root development.