CAJ | 학술논문

目的探讨白细胞介素-2(IL-2)基因多态性与慢性乙型肝炎的相关性,以及对HBVDNA水平的影响.方法应用聚合酶链反应-限制性片段长度多态性分析和DNA测序的方法检测155例慢性乙型肝炎患者和170名健康对照者的IL-2基因-385T/G、+114T/G单核苷酸多态性位点基因型,血清HBV DNA测定采用荧光定量PCR技术.结果 IL-2基因+114T/G多态性在慢性乙型肝炎组和正常人群中的频率分布差异无统计学意义.IL-2基因-385T/G多态性在两组人群中的频率分布差异有统计学意义,X2=7.377,P<0.05.等位基因频率的相对风险分析发现,G等位基因携带者患慢性乙型肝炎的风险是T等位基因的1.490倍(OR=1.490,95%CI:1.085-2.046);进一步比较慢性乙型肝炎患者IL一2基因多态性与HBV DNA复制的关系,发现高水平HBVDNA(≥1×10<'3>拷贝/ml)组-385G等位基因携带者分布频率明显高于低水平HBV DNA组(<1×10<'3>拷贝/ml),X2=6.051,P=0.014,差异有统计学意义.结论 IL-2基因-385T/G多态性可能与HBV具有相关性,其中G等位基因可能是慢性乙型肝炎的遗传易感基因,携带G等位基因的个体可能更利于HBV DNA的复制.
목적 탐토백세포개소-2(IL-2)기인다태성여만성을형간염적상관성,이급대HBVDNA수평적영향.방법 응용취합매련반응-한제성편단장도다태성분석화DNA측서적방법검측155례만성을형간염환자화170명건강대조자적IL-2기인-385T/G、+114T/G단핵감산다태성위점기인형,혈청HBV DNA측정채용형광정량PCR기술.결과 IL-2기인+114T/G다태성재만성을형간염조화정상인군중적빈솔분포차이무통계학의의.IL-2기인-385T/G다태성재량조인군중적빈솔분포차이유통계학의의,X2=7.377,P<0.05.등위기인빈솔적상대풍험분석발현,G등위기인휴대자환만성을형간염적풍험시T등위기인적1.490배(OR=1.490,95%CI:1.085-2.046);진일보비교만성을형간염환자IL일2기인다태성여HBV DNA복제적관계,발현고수평HBVDNA(≥1×10<'3>고패/ml)조-385G등위기인휴대자분포빈솔명현고우저수평HBV DNA조(<1×10<'3>고패/ml),X2=6.051,P=0.014,차이유통계학의의.결론 IL-2기인-385T/G다태성가능여HBV구유상관성,기중G등위기인가능시만성을형간염적유전역감기인,휴대G등위기인적개체가능경리우HBV DNA적복제.
Objectives To investigate the relationships of polymorphisms of interleukin-2 (IL-2) gone to the susceptibility of chronic hepatitis B, and to analyze the frequencies of 1L-2 gone polymorphisms and the HBV-DNA copies in patients with chronic hepatitis B. Methods Two single nueleotide polymor-phisms of IL-2 gone promoter-385T/G and +114T/G at exon one were analyzed in 155 patients with chronic hepatitis B and in 170 age- and sex-watched controls from a Chinese population. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy and DNA sequencing were used, and the serum HBV DNA in these patients was determined by real-time PCR. Results There were no differences in the distributions of IL-2 gone +114T/G polymorphism in the patient group and the controls, but their IL-2 gone -385T/G polymorphisms were significantly different (P < 0.05). The relative risk of being chronic hepa- titis B victims with T allele was 1.490 times of those with G allele (OR = 1.490, 95% CI: 1.085-2.046). The association between the genotype of 1L-2 gone polymorphisms and HBV-DNA copies showed that the frequency of-385G allele carriers in high HBV-DNA copy group was closer than that in low HBV-DNA copy group (X2 =6.051, P = 0.014). Conclusions IL-2 gone -385T/G polymorphism is assocated with chronic hepatitis B and G allele is an important genetic susceptibility gone for chronic hepatitis B. In IL-2 gene -385G allele carriers the risk of increasing HBV-DNA copies may be related to the pathogenesis of chronic hepatitis B.