CAJ | 학술논문

目的分析健康家兔眼轮匝肌(orbicularis oculi muscle,OOM)不同运动模式下的肌电信号特征,明确肌肉功能与肌电之间的关系,以期获得OOM肌电控制信号的信息,为人工面神经假体恢复单侧周围性面神经麻痹患者的闭眼功能提供参考.方法将微电极经上、下眼睑植入健康家兔的OOM内,采集OOM的肌电信号,并从时域、频域两方面分析信号特征.结果自然持续睁眼、自然持续闭眼、自然眨眼、诱发闭眼模式下OOM肌电的峰振幅分别为(28.8±4.8)μV、(36.0±4.7)μV、(398.8±195.7)μV和(715.4±249.7)μV,4种模式下的肌电信号功率谱(power spectrum density,PSD)峰频率分别为(98±17)Hz、(142±22)Hz、(203±58)Hz和(349±81)Hz.肌电活动在自然睁眼及自然闭眼时较平稳、幅值较低;自然眨眼及诱发闭眼时,肌电幅值明显增强,且诱发闭眼时肌电振幅增强程度较自然眨眼时强烈.持续睁眼与持续闭眼时肌电峰值绝对值均小于50 μV;自发眨眼及诱发闭眼动作发生时的肌电起始幅值绝对值在50～60 μV之间.OOM肌电PSD能量分布的整体频带为0～500 Hz,主要集中频带为20～350 Hz.在时域内,持续睁眼与持续闭眼OOM的肌电信号峰值绝对值差异无统计学意义(P＞0.05);其他各模式下峰值绝对值的两两比较差异均有统计学意义(P值均＜0.05).在频域内,持续睁眼与持续闭眼OOM肌电信号PSD峰频率的差异无统计学意义(P＞0.05);其他各模式下PSD峰频率间的两两比较差异均有统计学意义(P值均＜0.05).结论睁眼时OOM处于舒张状态,眼睑闭合动作是OOM收缩的结果.OOM肌电信号在不同运动模式下各具特点,可作为计算机判断识别此肌运动模式的依据,但根据时域和频域内OOM肌电特征很难将自然持续睁眼和自然持续闭眼状态进行区分.
목적 분석건강가토안륜잡기(orbicularis oculi muscle,OOM)불동운동모식하적기전신호특정,명학기육공능여기전지간적관계,이기획득OOM기전공제신호적신식,위인공면신경가체회복단측주위성면신경마비환자적폐안공능제공삼고.방법 장미전겁경상、하안검식입건강가토적OOM내,채집OOM적기전신호,병종시역、빈역량방면분석신호특정.결과 자연지속정안、자연지속폐안、자연잡안、유발폐안모식하OOM기전적봉진폭분별위(28.8±4.8)μV、(36.0±4.7)μV、(398.8±195.7)μV화(715.4±249.7)μV,4충모식하적기전신호공솔보(power spectrum density,PSD)봉빈솔분별위(98±17)Hz、(142±22)Hz、(203±58)Hz화(349±81)Hz.기전활동재자연정안급자연폐안시교평은、폭치교저;자연잡안급유발폐안시,기전폭치명현증강,차유발폐안시기전진폭증강정도교자연잡안시강렬.지속정안여지속폐안시기전봉치절대치균소우50 μV;자발잡안급유발폐안동작발생시적기전기시폭치절대치재50～60 μV지간.OOM기전PSD능량분포적정체빈대위0～500 Hz,주요집중빈대위20～350 Hz.재시역내,지속정안여지속폐안OOM적기전신호봉치절대치차이무통계학의의(P＞0.05);기타각모식하봉치절대치적량량비교차이균유통계학의의(P치균＜0.05).재빈역내,지속정안여지속폐안OOM기전신호PSD봉빈솔적차이무통계학의의(P＞0.05);기타각모식하PSD봉빈솔간적량량비교차이균유통계학의의(P치균＜0.05).결론 정안시OOM처우서장상태,안검폐합동작시OOM수축적결과.OOM기전신호재불동운동모식하각구특점,가작위계산궤판단식별차기운동모식적의거,단근거시역화빈역내OOM기전특정흔난장자연지속정안화자연지속폐안상태진행구분.
Objective To study the features of electromyogaphic signals of orbicularis oculi muscle (OOM) of normal rabbits in various movement states, and to clarify relationships between functional actions of OOM and their electromyographic signals, hoping to obtain information concerning the electromyographic signals controlling OOM as reference for restoring the eye-closing function by artificial facial nerve prosthesis in patients with unilateral peripheral facial palsy. Methods The electromyographic signals were extracted from OOM of normal rabbits by implanted microelectrodes through upper and lower eyelids. Then the features of these electromyographic signals were analyzed in the time domain and the frequency domain. Results The peak values of the absolute electromyographic amplitude for natural continuous eye-opening event,natural continuous eye-closing state, natural eye-blinking movement and evoked eye-closing state were (28. 8 ± 4. 8) μV, (36. 0 ± 4. 7 ) μV, ( 398. 8 ± 195.7 ) μV, and ( 715.4 ± 249. 7 ) μV, respectively. The peak frequency values of the power spectrum density (PSD) of electromyographic signals for the four modes were(98 ± 17 ) Hz, ( 142 ±22) Hz, (203 ±58) Hz, and( 349 ± 81 ) Hz, respectively. The electrical activities during the natural continuous eye-opening event and the natural continuous eye-closing state were stable and displayed low amplitudes. During the spontaneous blink state and the evoked eye-closing state, the electromyographic amplitudes markedly increased, and the increased level in the latter state was stronger than that in the former state. When rabbits continuously closed eyes or opened eyes, all of the peak values of thc absolute voltage amplitudes were less than 50 μV. The absolute amplitude values of the starting site were between 50 μV and 60 μV during the spontaneous blink and the evoked eye-closing movements. The whole frequency band of the energy of PSD about OOM was between 0 Hz and 500 Hz, and the focus frequency range was between 20 Hz and 350 Hz. In the time domain, the difference was not significant for the electromyographic signals of OOM between the continuous eye-opening state and the continuous eye-closing movement ( P ＞ 0. 05 ), but there were statistically significant differences in the other states for their pairwise comparisons( P ＜0. 05). In the frequency domain, there was no statistically significant difference for the peak frequency of PSD about the electromyographic signals when comparing the continuous eye-opening state with the continuous eye-closing event( P ＞ 0. 05 ). When comparing this item in the other movements with each other, however, the differences were statistically significant ( P ＜ 0. 05 ). Conclusions OOM relaxes when eyelid keeps continuously opening. The action of eyelid-closing is due to contraction of this muscle.Each state has its own features of the electromyographic signals for OOM, these features can be used as criteria for computers to judge and identify various movement states of OOM. However, it is difficult to distinguish the natural continuous eye-opening event from the natural continuous eye-closing state, based on the features of electromyographic signals in the time and frequency domain.