CAJ | 학술논문

目的检测喉鳞状细胞癌(简称鳞癌)组织缺氧诱导因子-10α( hypoxia inducible factor 1 alpha,HIF-1α)、葡萄糖转运蛋白-1(glucose transporter protein-1,GLUT-1)、血管内皮生长因子( vascular endothelial growth factor,VEGF)的蛋白表达情况,并通过体外实验进一步验证低氧对喉鳞癌细胞HIF-1α及其下游靶基因GLUT-1、VEGF的表达上调方面的重要促进作用.方法采用SP免疫组织化学和免疫细胞化学方法检测喉鳞癌组织和Hep-2细胞中HIF-1α、GLUT-1、VEGF蛋白的表达,分析HIF-1α与GLUT-1、VEGF表达的相关性.结果 35例喉癌患者组织切片中16例HIF-1α表达阳性(45.7％),16例GLUT-1表达阳性(阳性率45.7％),19例VEGF表达阳性(阳性率54.3％).HIF-1α和VEGF的表达水平与喉癌病理分级和淋巴转移有关(P值均＜0.05)；GLUT-1表达水平与淋巴转移有关(P＜0.05).体外实验结果显示,Hep-2细胞在低氧条件下HIF-1α、GLUT-1、VEGF的蛋白表达明显增加(P值均＜0.05).结论 HIF-1α可作为转录调控因子参与调控GLUT-1、VEGF的表达,进而在喉癌的发生、侵袭、转移过程中发挥重要作用.
목적 검측후린상세포암(간칭린암)조직결양유도인자-10α( hypoxia inducible factor 1 alpha,HIF-1α)、포도당전운단백-1(glucose transporter protein-1,GLUT-1)、혈관내피생장인자( vascular endothelial growth factor,VEGF)적단백표체정황,병통과체외실험진일보험증저양대후린암세포HIF-1α급기하유파기인GLUT-1、VEGF적표체상조방면적중요촉진작용.방법 채용SP면역조직화학화면역세포화학방법검측후린암조직화Hep-2세포중HIF-1α、GLUT-1、VEGF단백적표체,분석HIF-1α여GLUT-1、VEGF표체적상관성.결과 35례후암환자조직절편중16례HIF-1α표체양성(45.7％),16례GLUT-1표체양성(양성솔45.7％),19례VEGF표체양성(양성솔54.3％).HIF-1α화VEGF적표체수평여후암병리분급화림파전이유관(P치균＜0.05)；GLUT-1표체수평여림파전이유관(P＜0.05).체외실험결과현시,Hep-2세포재저양조건하HIF-1α、GLUT-1、VEGF적단백표체명현증가(P치균＜0.05).결론 HIF-1α가작위전록조공인자삼여조공GLUT-1、VEGF적표체,진이재후암적발생、침습、전이과정중발휘중요작용.
Objective To detect the expression of hypoxia inducible factor 1 alpha (HIF-1α),glucose transporter protein-1 (GLUT-1) and vascular endothelial growth factor (VEGF) in human laryngeal carcinoma tissue,and to study the relationship between hypoxia and HIF-1α,GLUT-1,VEGF in human laryngeal carcinoma Hep-2 cells and to explore the effect of HIF-1α,GLUT-1 and VEGF as endogenous hypoxic markers on laryngeal carcinoma.Methods The expression levels of HIF-1α,GLUT-1 and VEGF were detected in 35 cases of laryngeal carcinoma by SP immunohistochemical methods and in Hep-2 cells by SP immunocytochemical methods.The relationship between HIF-1 α and GLUT-1,VEGF protein expression was analyzed.Results Of the 35 cases,16 cases expressed HIF-1α,16 cases expressed GLUT-1,19 cases expressed VEGF.The expression of HIF-1α and VEGF were closely correlated with pathologic grading and lymphnode metastasis.GLUT-1 was correlated with lymphnode metastasis.The expression levels of HIF-1 α,GLUT-1 and VEGF in Hep-2 cells under hypoxic condition were higher than those under normoxiccondition.Conclusion HIF-1α may promote the expression of GLUT-1 and VEGF in laryngeal carcinoma,furthermore promote tumor angiogenesis,invasion,and metastasis of the laryngeal carcinoma.