CAJ | 학술논문

目的探讨点阵铒:钇铝石榴石(Er:YAG)激光定点多重脉冲照射对正常人皮肤的组织学影响.方法使用点阵Er:YAG激光连续多脉冲照射正常受试者的上臂皮肤,取照射后1h、24h、48 h、72 h、7d、15d及30 d的照射皮肤和未照射皮肤进行组织学观察,包括HE染色、抗热休克蛋白70、47免疫组化染色和Masson染色.结果点阵Er:YAG激光连续多脉冲照射正常受试者皮肤,刺激皮肤组织产生一系列点阵排列的局灶性锥状气化剥脱微损伤.照射后1～7 d,微损伤区皮肤产生炎症反应,表皮细胞上皮化；7～30d局部真皮内胶原新生、重塑.热休克蛋白70在照射后72 h至第7天内呈峰值表达；热休克蛋白47在照射后15～30 d持续高表达.结论点阵Er:YAG激光定点多重脉冲照射人体皮肤可以到达真皮深层,从而获得局灶性胶原增生的疗效.
목적 탐토점진이:을려석류석(Er:YAG)격광정점다중맥충조사대정상인피부적조직학영향.방법 사용점진Er:YAG격광련속다맥충조사정상수시자적상비피부,취조사후1h、24h、48 h、72 h、7d、15d급30 d적조사피부화미조사피부진행조직학관찰,포괄HE염색、항열휴극단백70、47면역조화염색화Masson염색.결과 점진Er:YAG격광련속다맥충조사정상수시자피부,자격피부조직산생일계렬점진배렬적국조성추상기화박탈미손상.조사후1～7 d,미손상구피부산생염증반응,표피세포상피화；7～30d국부진피내효원신생、중소.열휴극단백70재조사후72 h지제7천내정봉치표체；열휴극단백47재조사후15～30 d지속고표체.결론 점진Er:YAG격광정점다중맥충조사인체피부가이도체진피심층,종이획득국조성효원증생적료효.
Objective To investigate the histological changes of normal human skin after fixed irradiation with multiple-pulse fractional Er:YAG laser.Methods After hair removal,the upper arms of 7 healthy volunteers were consecutively irradiated with multiple-pulse fractional Er:YAG laser.Tissue samples were resected from the irradiated skin at 1,24,48,72 hours,on day 5,7,15,and 30 after the irradiation.Hematoxylin and eosin(HE)staining and Masson staining were performed to observe the histological changes and collagen proliferation respectively.Immunohistochemical staining was used to detect the expression of heat shock protein(HSP)70 and 47.Results The consecutive irradiation with multi-pulse fractional Er:YAG laser generated an array of tapering microscopic treatment zones(MTZs)of gasification and ablation in the upper arm skin.After the irradiation,inflammation developed in the microscopic lesions with the epithelization of epidermal cells within 7 days; local dermal collagen was renewed and remodelled during the 7th to 30th day.HSP70 expression peaked as early as 48 hours after the treatment and maintained until the 7th day,while the high expression of HSP47 persisted from the 15th to 30th day after irradiation.Conclusion The fixed,multiplepulse and fractional Er:YAG laser irradiation can reach the deep dermis,and induce the local proliferation of dermal collagen.