中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
11期
816-819
,共4页
徐新云%刘月峰%易娟%柯跃斌%袁建辉%黄海燕%毛吉炎%毛侃琅
徐新雲%劉月峰%易娟%柯躍斌%袁建輝%黃海燕%毛吉炎%毛侃瑯
서신운%류월봉%역연%가약빈%원건휘%황해연%모길염%모간랑
三氯乙烯%皮炎,职业性%超敏反应%基因表达
三氯乙烯%皮炎,職業性%超敏反應%基因錶達
삼록을희%피염,직업성%초민반응%기인표체
Trichloroethylene%Dermatitis,Occupational exposure%Hypersensitivity%Gene expression
目的 探讨三氯乙烯(TCE)药疹样皮炎患者外周血免疫相关基因Foxp3、GATA3、CTLA4、T-bet的mRNA表达.方法 选取TCE药疹样皮炎患者8例为病例组,8例健康人为对照组,抽取两组对象的外周血,采用实时荧光定量PCR(real-time quantitative PCR)技术检测外周血免疫相关基因Foxp3、GATA3、CTLA4、T-bet的mRNA的表达水平.结果 与对照组比较,病例组Foxp3、GATA3、CT-LA4基因mRNA表达水平分别升高115％、97％和241％,差异有统计学意义(P＜0.01)；与对照组比较,病例组T-bet基因mRNA表达水平下降47％,差异有统计学意义(P＜0.01).结论 TCE药疹样皮炎的部分免疫相关基因表达水平发生明显改变,这些基因可能在TCE引起的变态反应中发挥重要作用.
目的 探討三氯乙烯(TCE)藥疹樣皮炎患者外週血免疫相關基因Foxp3、GATA3、CTLA4、T-bet的mRNA錶達.方法 選取TCE藥疹樣皮炎患者8例為病例組,8例健康人為對照組,抽取兩組對象的外週血,採用實時熒光定量PCR(real-time quantitative PCR)技術檢測外週血免疫相關基因Foxp3、GATA3、CTLA4、T-bet的mRNA的錶達水平.結果 與對照組比較,病例組Foxp3、GATA3、CT-LA4基因mRNA錶達水平分彆升高115％、97％和241％,差異有統計學意義(P＜0.01)；與對照組比較,病例組T-bet基因mRNA錶達水平下降47％,差異有統計學意義(P＜0.01).結論 TCE藥疹樣皮炎的部分免疫相關基因錶達水平髮生明顯改變,這些基因可能在TCE引起的變態反應中髮揮重要作用.
목적 탐토삼록을희(TCE)약진양피염환자외주혈면역상관기인Foxp3、GATA3、CTLA4、T-bet적mRNA표체.방법 선취TCE약진양피염환자8례위병례조,8례건강인위대조조,추취량조대상적외주혈,채용실시형광정량PCR(real-time quantitative PCR)기술검측외주혈면역상관기인Foxp3、GATA3、CTLA4、T-bet적mRNA적표체수평.결과 여대조조비교,병례조Foxp3、GATA3、CT-LA4기인mRNA표체수평분별승고115％、97％화241％,차이유통계학의의(P＜0.01)；여대조조비교,병례조T-bet기인mRNA표체수평하강47％,차이유통계학의의(P＜0.01).결론 TCE약진양피염적부분면역상관기인표체수평발생명현개변,저사기인가능재TCE인기적변태반응중발휘중요작용.
Objective To study mRNA expression of immune-related genes (Foxp3,GATA3,CTLA4 and T-bet) in peripheral blood of the patients with allergic dermatitis induced by trichloroethylene (TCE).Methods The peripheral blood samples were collected from 8 healthy workers (control group) and 8 patients with allergic dermatitis induced by TCE (case group).Real-time quantitative PCR was applied to detect mRNA expression of immune-related genes (Foxp3,GATA3,CTLA4,T-bet).Results The mRNA expression levels of Foxp3,GATA3 and CTLA4 genes increased by 115％,97％ and 241％ in case group,as compared with control group (P＜0.01).The mRNA expression level of T-bet gene decreased by 47％ in case group,as compared with control group (P＜0.01).Conclusion The mRNA expression levels of some immune-related genes changed in patients with allergic dermatitis induced by TCE,those genes may play an important role in TCE-induced allergy.
