CAJ | 학술논문

目的探讨雌激素受体拮抗剂4-羟基他莫昔芬(OHTam)对泌乳素腺瘤GH3细胞中垂体瘤转化基因(PTTG)表达的影响. 方法采用逆转录多聚酶链式反应(RT-PCR)和Westernblot法,测定GH3细胞中PTTG mRNA和PTTG蛋白表达.在去激素培养条件下观察不同浓度的4-羟基他莫昔芬(OHTam)和雌二醇(E2)对细胞生长速度、PTTG mRNA和PTTG蛋白表达水平的影响.结果 GH3细胞在去激素环境下细胞生长明显减慢,低浓度E2(1×10<'-8>mol/L)可促进其生长,OHTam(1×10<'-6>mol/L)可抑制E2(1×10<'-8>mol/L)的生长刺激作用;GH3细胞中存在PTTGmRNA和PTTG蛋白的表达,且OHTam(1×10<'-6>mol/L)可抑制它们的表达水平.结论泌乳素腺瘤GH3细胞的生长具有雌激素依赖性,应用雌激素受体拮抗剂OHTam能抑制GH3细胞生长和PTTG原癌基因的表达水平.
목적 탐토자격소수체길항제4-간기타막석분(OHTam)대비유소선류GH3세포중수체류전화기인(PTTG)표체적영향. 방법채용역전록다취매련식반응(RT-PCR)화Westernblot법,측정GH3세포중PTTG mRNA화PTTG단백표체.재거격소배양조건하관찰불동농도적4-간기타막석분(OHTam)화자이순(E2)대세포생장속도、PTTG mRNA화PTTG단백표체수평적영향.결과 GH3세포재거격소배경하세포생장명현감만,저농도E2(1×10<'-8>mol/L)가촉진기생장,OHTam(1×10<'-6>mol/L)가억제E2(1×10<'-8>mol/L)적생장자격작용;GH3세포중존재PTTGmRNA화PTTG단백적표체,차OHTam(1×10<'-6>mol/L)가억제타문적표체수평.결론비유소선류GH3세포적생장구유자격소의뢰성,응용자격소수체길항제OHTam능억제GH3세포생장화PTTG원암기인적표체수평.
Objective To investigate the effect of 4-hydroxytamoxifen on the expression of pituitary tumor transforming gene (PTTG) in GH3 prolactinoma cells. Methods RT-PCR and Western blotting were employed to detect the expressions of PTTG mRNA and protein in human GH3 prolaetinoma cells. Different concentrations of estradiol (E2) or 4-hydroxytamoxifen (OHTam) were addedl into the hormone-depleted medium, and the viable cell number and expression levels of PTTG mRNA and protein were measured. Results The growth of OH3 prolaetinoma cells was significantly inhibited in hormone-depleted medium. E2 at a concentration of 1×10<'-8> mol/L obviously promoted the cell growth, the effect of which was inhibited by the application of OHTam (1×10<'-6> mol/L) to cause slowed cell growth. The expressions of PTTG at both the mRNA and protein levels were detected in detected in untreated GH3 prolactinoma cells, and OHTam at the concentration of 1×10<'-6> mol/L significantly inhibited their expressions. Conclusion The growth of GH3 cells is estrogen-dependant and can be inhibited by estrogen antagonist OHTam, which also results in reduced expression of PTTG gene in the cells.