CAJ | 학술논문

目的检测脑肿瘤干细胞(BTSC)标记物CD133、巢蛋白(Nestin)和增殖细胞核抗原(PCNA)在74例脑胶质瘤标本中的表达,探讨肿瘤干细胞生存的微环境一壁龛的组成、形态及其在脑肿瘤组织中的分布. 方法选取安徽医科大学附属省立医院神经外科自2007年1月至2008年10月间手术切除的74例胶质瘤标本,按照WH02000年的神经系统肿瘤分类分级标准分为Ⅱ级22例(低级别组)、Ⅲ级27例和Ⅳ级25例(高级别组),采用免疫组织化学染色和免疫荧光双标法分别检测标本中CD133的表达及其与Nestin、PCNA的共表达情况.计算并比较不同级别胶质瘤组织CD133+细胞、CD133+血管和CD133+壁龛所占的百分比.并对CD133+血管和CD133+壁龛的表达进行相关性分析. 结果 CD133+细胞聚集于壁龛内生长,低级别组胶质瘤中CD133+壁龛阳性率较低.壁龛内增殖细胞较少,与相邻肇龛之间界限清晰,周围CD133+血管分布较少.高级别组胶质瘤中CD133+壁龛阳性率高,壁龛之间无明显界限,壁龛内细胞增殖活跃,周围可见丰富的CD133+血管分布:壁龛中除CD133+/Nestin+BTSC外,可见CD133+/Nestin-细胞、CD133/PCNA+细胞等亚群细胞;不同级别胶质瘤CD133+细胞、CD133+血管、CD133+壁龛百分比不同,且肿瘤级别越高,三者表达越高,差异有统计学意义(P<0.05).CD133+壁龛与CD133+血管的表达呈正相关(r=0.425,P=0.000). 结论在脑胶质瘤组织中存在着由CD133+/Nestin+BTSC和一些亚群细胞组成的壁龛结构,CD133+血管对于壁龛结构的维持起着非常重要的作用.
목적 검측뇌종류간세포(BTSC)표기물CD133、소단백(Nestin)화증식세포핵항원(PCNA)재74례뇌효질류표본중적표체,탐토종류간세포생존적미배경일벽감적조성、형태급기재뇌종류조직중적분포. 방법 선취안휘의과대학부속성립의원신경외과자2007년1월지2008년10월간수술절제적74례효질류표본,안조WH02000년적신경계통종류분류분급표준분위Ⅱ급22례(저급별조)、Ⅲ급27례화Ⅳ급25례(고급별조),채용면역조직화학염색화면역형광쌍표법분별검측표본중CD133적표체급기여Nestin、PCNA적공표체정황.계산병비교불동급별효질류조직CD133+세포、CD133+혈관화CD133+벽감소점적백분비.병대CD133+혈관화CD133+벽감적표체진행상관성분석. 결과 CD133+세포취집우벽감내생장,저급별조효질류중CD133+벽감양성솔교저.벽감내증식세포교소,여상린조감지간계한청석,주위CD133+혈관분포교소.고급별조효질류중CD133+벽감양성솔고,벽감지간무명현계한,벽감내세포증식활약,주위가견봉부적CD133+혈관분포:벽감중제CD133+/Nestin+BTSC외,가견CD133+/Nestin-세포、CD133/PCNA+세포등아군세포;불동급별효질류CD133+세포、CD133+혈관、CD133+벽감백분비불동,차종류급별월고,삼자표체월고,차이유통계학의의(P<0.05).CD133+벽감여CD133+혈관적표체정정상관(r=0.425,P=0.000). 결론 재뇌효질류조직중존재착유CD133+/Nestin+BTSC화일사아군세포조성적벽감결구,CD133+혈관대우벽감결구적유지기착비상중요적작용.
Objective To investigate the expressions of the putative brain tumor stem cell (BTSC) marker CD133, nestin and proliferating cell nuclear antigen (PCNA) in formalin-fixed and paraffin-embedded gliomas, and discuss the BTSC microenvironment: the composition, morphology and distribution of the niche. Methods The samples of 74 patients performed resection of the glioma in our hospital from January 2007 and October 2008 were chosen. According to WHO 2008 classification of nervous system tumors, they were assigned into low-grade glioma group (grade Ⅱ, 22) and high-grade glioma group(grade Ⅲ, 27 and grade Ⅳ,25). Immunohistochemistry was used to detect the expression of CD 133 in 74 cases of brain gliomas. Double immunofluorescence staining was employed to detect the co-expressions of CD133/nestin or CD133/PCNA. The percentages of the CD133+cells, CD133+ blood vessels and CD 133+ niches were calculated, and correlation analysis was also performed on their percentage and the pathological grading of the tumor. Results Accumulated CD 133+ cells grew in the niches. CD 133+ niches could be observed in all grade gliomas; low-grade group showed lower level expression of the CD 133+ niches, fewer proliferating cells, clearer boundary between the 2 neighboring niches and fewer surrounding blood vessels as compared with high-grade group. Besides CD113+/nestin+ BTSCs, some subsets as CD133+/nestin-cells and CD133-/PCNA+ cells were noted in the niches in the high-grade group. The percentages of CD 133+ cells, CD 133+ blood vessels and CD 133+ niches were different in different grades of gliomas and the higher the grade of gliomas was, the higher their expressions were. Positive correlation was observed between the percentage of CD 133+ niches and the percentage of CD133+ blood vessels (r=0.425, P=0.000). The expression of CD133+ niches in high-grade glioma were higher than that in low-grade tumors (F=5.324, P=0.002). Conclusion Glioma tissues have niche structures, which composed of CD133+/nestin+ BTSCs and some subsets. CD133+ blood vessels were playing a key role in maintaining the niche structure, and the expression of CD133+ niches is statistically different in different pathological grading tumors.