中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2012年
3期
165-167
,共3页
闫晓%马晶%张晓梅%常战军%刘建勋%兰琳%徐兰英%张胜勇%白建敏%钱建华%张晓光%曾毅
閆曉%馬晶%張曉梅%常戰軍%劉建勛%蘭琳%徐蘭英%張勝勇%白建敏%錢建華%張曉光%曾毅
염효%마정%장효매%상전군%류건훈%란림%서란영%장성용%백건민%전건화%장효광%증의
HIV%基因%横断面研究%药物耐受,病毒
HIV%基因%橫斷麵研究%藥物耐受,病毒
HIV%기인%횡단면연구%약물내수,병독
HIV%Genes%Cross sectional studies%Drug resisitance,viral
目的 了解河南省某市HIV耐药毒株在HIV/AIDS患者中的情况.方法 采集2011年河南省某市150份HIV/AIDS患者血清,提取RNA,逆转录及巢式PCR扩增HIV-1pol基因区,所得序列构建系统进化树分析亚型；并利用Stanford HIV Drug Resistance Database分析HIV基因耐药相关突变和耐药情况.结果 150份样本中34份测序阳性,其中未接受抗病毒治疗样本9份,接受抗病毒治疗样本25份；B’亚型占94.1％.测序阳性样本的基因突变率为58.8％,其中未接受抗病毒治疗的突变率为5.9％,接受抗病毒治疗的突变率为52.9％.结论 接受抗病毒治疗的耐药样本中出现了对反转录酶抑制剂不同程度的耐药及多药耐药,说明仍需加强耐药监测及管理,尽量减少耐药株的产生,并根据耐药检测结果及时调整治疗方案.
目的 瞭解河南省某市HIV耐藥毒株在HIV/AIDS患者中的情況.方法 採集2011年河南省某市150份HIV/AIDS患者血清,提取RNA,逆轉錄及巢式PCR擴增HIV-1pol基因區,所得序列構建繫統進化樹分析亞型；併利用Stanford HIV Drug Resistance Database分析HIV基因耐藥相關突變和耐藥情況.結果 150份樣本中34份測序暘性,其中未接受抗病毒治療樣本9份,接受抗病毒治療樣本25份；B’亞型佔94.1％.測序暘性樣本的基因突變率為58.8％,其中未接受抗病毒治療的突變率為5.9％,接受抗病毒治療的突變率為52.9％.結論 接受抗病毒治療的耐藥樣本中齣現瞭對反轉錄酶抑製劑不同程度的耐藥及多藥耐藥,說明仍需加彊耐藥鑑測及管理,儘量減少耐藥株的產生,併根據耐藥檢測結果及時調整治療方案.
목적 료해하남성모시HIV내약독주재HIV/AIDS환자중적정황.방법 채집2011년하남성모시150빈HIV/AIDS환자혈청,제취RNA,역전록급소식PCR확증HIV-1pol기인구,소득서렬구건계통진화수분석아형；병이용Stanford HIV Drug Resistance Database분석HIV기인내약상관돌변화내약정황.결과 150빈양본중34빈측서양성,기중미접수항병독치료양본9빈,접수항병독치료양본25빈；B’아형점94.1％.측서양성양본적기인돌변솔위58.8％,기중미접수항병독치료적돌변솔위5.9％,접수항병독치료적돌변솔위52.9％.결론 접수항병독치료적내약양본중출현료대반전록매억제제불동정도적내약급다약내약,설명잉수가강내약감측급관리,진량감소내약주적산생,병근거내약검측결과급시조정치료방안.
Objective To find the prevalence of genotype drug-resistance mutation in HIV/AIDS infected individuals in Henan.Methods Totally 150 plasma samples were collected for BNA extraction from HIV/AIDS infected individuals in Henan in 2011.The HIV-1pol geue region was amplified by the methods of reverse transcription and nested-PCR.The phylogenetic tree was constructed based on DNA sequences,and HIV genotype drug-resistance mutation was analyzed.Results Thirty-four of 150 plasma samples were successfully sequenced (9 in untreated group and 25 in antiretroviral therapy group).HIV-1clade B' was major genetic subtype,which accounted for 94.1％ respectively.The percentage of HIV genotype drugresistance samples in sequenced samples was 58.8％ (5.9％ in untreated group and 52.9％ in antiretroviral therapy group).Conclusion The samples with genotypic resistance in antiretroviral therapy group showed resistance to RT inhibitors incoordinately and multidrug resistance.Monitoring and management system of drug resistance should be improved.Treatment should be tried to reduce the generation of HIV drugresistance strains.And adjustment of the drug scheme should be in time.
