中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
8期
689-694
,共6页
表皮葡萄球菌%细菌生物被膜%cidA%lrgA
錶皮葡萄毬菌%細菌生物被膜%cidA%lrgA
표피포도구균%세균생물피막%cidA%lrgA
Staphylococcus epidermidis%Bacterial biofilm%cidA%lrgA
目的 cid和lrg操纵子是细菌程序性死亡和溶解的重要调控基因,本研究旨在探究表皮葡萄球菌临床株cidA和lrgA基因及其mRNA表达水平与生物被膜形成的关系.方法 前期收集的表皮葡萄球菌(来源于血液、尿液、痰、分泌物及导管等生物材料表面)临床株39株,通过黏附试验和icaA基因扩增将其分为生物被膜形成菌株20株,非生物被膜形成菌株19株,PCR扩增表皮葡萄球菌cidA和lrgA基因片段,半定量RT-PCR检测cidA和lrgA mRNA水平,计算cid/lrg mRNA比率.结果 39株表皮葡萄球菌临床株均可扩增出cidA和lrgA的基因片段.随机选取生物被膜形成菌株表皮葡萄球菌Y36和非生物被膜形成菌株表皮葡萄球菌Y26检测培养2、4、6、8、10 h的cidA和lrgA的表达水平,两株菌cidA mRNA均在培养4 h后达峰,lrgA mRNA均在培养6 h后达峰.39株表皮葡萄球菌培养4 h后,生物被膜形成菌株与非生物被膜形成菌株,菌株中cidA基因相对表达水平分别为0.340±0.250和0.406±0.408(P=0.541),lrgA基因相对表达水平为0.325±0.218和0.253±0.211(P=0.299),两组相比差异均无统计学意义.cid/lrg mRNA比率,生物被膜形成菌株为1.067±0.529,非生物被膜形成菌株为1.958±1.877,两组总体分布差异有统计学意义(P=0.001).结论 表皮葡萄球菌临床株cidA和lrgA基因表达可能具有一定的时限性,cidA和lrgA基因在生物被膜形成菌株与非生物被膜形成菌株表皮葡萄球菌的表达没有明显差异,cid/lrg mRNA比率可能具有一定生物学意义.
目的 cid和lrg操縱子是細菌程序性死亡和溶解的重要調控基因,本研究旨在探究錶皮葡萄毬菌臨床株cidA和lrgA基因及其mRNA錶達水平與生物被膜形成的關繫.方法 前期收集的錶皮葡萄毬菌(來源于血液、尿液、痰、分泌物及導管等生物材料錶麵)臨床株39株,通過黏附試驗和icaA基因擴增將其分為生物被膜形成菌株20株,非生物被膜形成菌株19株,PCR擴增錶皮葡萄毬菌cidA和lrgA基因片段,半定量RT-PCR檢測cidA和lrgA mRNA水平,計算cid/lrg mRNA比率.結果 39株錶皮葡萄毬菌臨床株均可擴增齣cidA和lrgA的基因片段.隨機選取生物被膜形成菌株錶皮葡萄毬菌Y36和非生物被膜形成菌株錶皮葡萄毬菌Y26檢測培養2、4、6、8、10 h的cidA和lrgA的錶達水平,兩株菌cidA mRNA均在培養4 h後達峰,lrgA mRNA均在培養6 h後達峰.39株錶皮葡萄毬菌培養4 h後,生物被膜形成菌株與非生物被膜形成菌株,菌株中cidA基因相對錶達水平分彆為0.340±0.250和0.406±0.408(P=0.541),lrgA基因相對錶達水平為0.325±0.218和0.253±0.211(P=0.299),兩組相比差異均無統計學意義.cid/lrg mRNA比率,生物被膜形成菌株為1.067±0.529,非生物被膜形成菌株為1.958±1.877,兩組總體分佈差異有統計學意義(P=0.001).結論 錶皮葡萄毬菌臨床株cidA和lrgA基因錶達可能具有一定的時限性,cidA和lrgA基因在生物被膜形成菌株與非生物被膜形成菌株錶皮葡萄毬菌的錶達沒有明顯差異,cid/lrg mRNA比率可能具有一定生物學意義.
목적 cid화lrg조종자시세균정서성사망화용해적중요조공기인,본연구지재탐구표피포도구균림상주cidA화lrgA기인급기mRNA표체수평여생물피막형성적관계.방법 전기수집적표피포도구균(래원우혈액、뇨액、담、분비물급도관등생물재료표면)림상주39주,통과점부시험화icaA기인확증장기분위생물피막형성균주20주,비생물피막형성균주19주,PCR확증표피포도구균cidA화lrgA기인편단,반정량RT-PCR검측cidA화lrgA mRNA수평,계산cid/lrg mRNA비솔.결과 39주표피포도구균림상주균가확증출cidA화lrgA적기인편단.수궤선취생물피막형성균주표피포도구균Y36화비생물피막형성균주표피포도구균Y26검측배양2、4、6、8、10 h적cidA화lrgA적표체수평,량주균cidA mRNA균재배양4 h후체봉,lrgA mRNA균재배양6 h후체봉.39주표피포도구균배양4 h후,생물피막형성균주여비생물피막형성균주,균주중cidA기인상대표체수평분별위0.340±0.250화0.406±0.408(P=0.541),lrgA기인상대표체수평위0.325±0.218화0.253±0.211(P=0.299),량조상비차이균무통계학의의.cid/lrg mRNA비솔,생물피막형성균주위1.067±0.529,비생물피막형성균주위1.958±1.877,량조총체분포차이유통계학의의(P=0.001).결론 표피포도구균림상주cidA화lrgA기인표체가능구유일정적시한성,cidA화lrgA기인재생물피막형성균주여비생물피막형성균주표피포도구균적표체몰유명현차이,cid/lrg mRNA비솔가능구유일정생물학의의.
Objective To investigate the relationships between the expressions of cidA and lrgA and the biofilm formation of Staphylococcus epidermidis clinical isolates. Methods Thirty-nine S. epidermidis clinical isolates were divided into biofilm formation group and non-biofilm formation group according to adhesion assays and icaA amplification, 20 strains for biofilm formation group and 19 strains for another group respectively. Amplify cidA and lrgA fragment with PCR, detect mRNA levels of cidA and lrgA with RT-PCR and then calculate the ratios of cid/lrg mRNA. Results All of the 39 isolates of S. epidermidis existed cidA and lrgA gene.Select 2 strains of S. epidermidis, Y36 and Y26, at random for detecting the cidA and lrgA mRNA levels of different phases, the highest level of cidA mRNA shows on 4 h's culture while lrgA shows on6 h. The relative expression levels of cidA of 39 isolates of S. epidermidis were 0.340 + 0.250 in biofilm formation group and 0. 406 +0. 408( P =0. 541 ) in non-biofilm formation group for 4 h culture, while the relative expression levels of lrgA were 0.325 ± 0.218 and 0.253 ± 0.211 respectively ( P = 0. 299). There were no significant differences between these two groups. The ratios of cid/lrg mRNA were 1.067 ±0.529 and 1.958 ±1.877 respectively, the difference of population distribution was significant( P = 0.001 ). Conclusion The expressions of cidA and lrgA of S. epidermidis clinical isolates may be time-limited. There were no significant differeces of expressions of cidA and lrgA between biofilm formation group and non-biofilm formation group. The ratios of cid/lrg mRNA maybe have some biological significance.
