CAJ | 학술논문

本文采用大鼠乳头肌张力测定及离体心脏灌流技术,研究大鼠心肌Na+-Ca2+交换对乳头肌及离体灌流心脏变力性的影响.采用大鼠特异性Na+-Ca2+交换激动剂E-4031能剂量依赖性地增加大鼠乳头肌的发展张力(P＜0.05,n=6)及离体心脏的心泵功能(P＜0.05,n=4);特异性Na+-Ca2+交换抑制剂KB-R7943具有相反的效应,并可完全消除E-4031引起的正性变力作用.哇巴因(ouabain,0.5μmol/L)与E-4031(3μmol/L)联合使用,可使乳头肌发展张力由单独使用哇巴因时的0.25±0.03 g升高至0.29±0.04g(P＜0.05,n=6);联合用药对大鼠离体心脏心泵功能的影响也强于哇巴因单独作用的效果.本研究结果证实,E-4031通过增强心肌Na+-Ca2+交换,对大鼠乳头肌和离体心脏产生正性变力作用;与哇巴因合用时,它们的正性变力作用有相加作用.
본문채용대서유두기장력측정급리체심장관류기술,연구대서심기Na+-Ca2+교환대유두기급리체관류심장변력성적영향.채용대서특이성Na+-Ca2+교환격동제E-4031능제량의뢰성지증가대서유두기적발전장력(P＜0.05,n=6)급리체심장적심빙공능(P＜0.05,n=4);특이성Na+-Ca2+교환억제제KB-R7943구유상반적효응,병가완전소제E-4031인기적정성변력작용.왜파인(ouabain,0.5μmol/L)여E-4031(3μmol/L)연합사용,가사유두기발전장력유단독사용왜파인시적0.25±0.03 g승고지0.29±0.04g(P＜0.05,n=6);연합용약대대서리체심장심빙공능적영향야강우왜파인단독작용적효과.본연구결과증실,E-4031통과증강심기Na+-Ca2+교환,대대서유두기화리체심장산생정성변력작용;여왜파인합용시,타문적정성변력작용유상가작용.
To study the inotropic effect of enhanced Na+-Ca2+ exchange in the rat papillary muscles and isolated heart, the developed tension in the rat papillary muscles was measured and the left ventricular functions were assessed in the isolated rat heart. E-4031, a selective activator for Na+-Ca2+ exchange in rats, concentration-dependently increased the developed contractile tension in the rat papillary muscles (P＜0.05, n=6) and the left ventricular functions in the isolated heart; KB-R7943, a selective Na+-Ca2+ exchange inhibitor,exhibited opposite effect. A combination of 0.5 μmol/L ouabain and 3.0 μmol/L E-4031 resulted in a potentiation of the developed contractile tension of the rat papillary muscles from 0.25±0.03 g to 0.29±0.04 g. The combination also enhanced the augmentation of the left ventricular functions induced by ouabain. These results indicate that E-4031 exerts a positive inotropic effect on the rat papillary muscles and isolated heart via increasing the activity of Na+-Ca2+ exchange, and potentiates the positive inotropic effects of ouabain.