背景:作为胞浆中的一种生化标志物,人精子中肌酸激酶活性增高与精浆残余量和异常功能精子比例二者有关.它即是成熟精子的标志,又与受精能力相关.目的:观察精子肌酸激酶活性及精子M-肌酸激酶、B-肌酸激酶同工酶的相对含量在健康生育男性和精子健康男性精子中的差异,评价其在男性精子健康临床诊断中的意义.设计:病例-对照观察.单位:四川省计划生育科学研究所生化室.对象:于1999-01/2000-10选择四川省计划生育科学研究所专科门诊收治男性精子健康患者94例,已排除女方不孕原因和无精子症,平均年龄31岁.精子计数＞2×1010 L-1的精子健康男性80例,为精子正常组,精子计数＜2×1010 L-1的精子健康男性患者14例,为少精组.同期精液常规检查完全健康且已有后代的健康男性供精者27例,为健康对照组.方法:所有对象禁欲3～5 d,用手淫法采集精液,经37℃液化后进行精液常规检查.酶动力学分光光度法测定精子肌酸激酶总活性,琼脂糖电泳凝胶光密度扫描测定肌酸激酶的同工酶相对含量.主要观察指标:精子计数,精子存活率,运动精子百分比,精子肌酸激酶总活性和M-肌酸激酶及B-肌酸激酶同工酶的相对含量.结果:纳入患者94例和健康对照者27例,均进入结果分析.①少精组的精子计数,精子存活率和运动精子百分比(Ⅱ+Ⅲ,WHO分类法)均显著低于健康对照组,精子正常组除精子计数外,其他指标也显著低于健康对照组[健康对照组、精子正常组、少精组精子计数分别为(6.05±0.81)×1010 L-1,(7.76±1.37)×1010 L-1,(1.46±0.19)×1010 L-1,P＜0.01;健康对照组、精子正常组、少精组精子存活率分别为85.1%,56.8%,58.2%,P＜0.01;健康对照组、精子正常组、少精组运动精子百分比(Ⅱ+Ⅲ)分别为62.9%,34.6%,29.5%,P＜0.01].②少精组肌酸激酶活性显著高于健康对照组(少精组、健康对照组每1×108精子中肌酸激酶活性分别为(9.000±6.117),(1.933±0.943)kat,P＜0.05),精子正常组每1×108精子中肌酸激酶活性为(2.800±0.862)kat,虽略高于健康对照组,但两者比较差异无显著性(P＞0.05).③精子正常组和少精组M-肌酸激酶同工酶含量都显著减少(健康对照组、精子正常组、少精组分别为30.5%,19.0%,18.0%,P＜0.05),提示两组患者的精子分化程度与健康对照组精子分化程度可能有明显不同.结论:精子肌酸激酶活性测定对少精子症的诊断和病因分析具有一定的意义.精子M-肌酸激酶同工酶相对含量在不同精子健康男性群体中的分布规律及其在男性精子健康诊断中的作用有待更深入的研究.
揹景:作為胞漿中的一種生化標誌物,人精子中肌痠激酶活性增高與精漿殘餘量和異常功能精子比例二者有關.它即是成熟精子的標誌,又與受精能力相關.目的:觀察精子肌痠激酶活性及精子M-肌痠激酶、B-肌痠激酶同工酶的相對含量在健康生育男性和精子健康男性精子中的差異,評價其在男性精子健康臨床診斷中的意義.設計:病例-對照觀察.單位:四川省計劃生育科學研究所生化室.對象:于1999-01/2000-10選擇四川省計劃生育科學研究所專科門診收治男性精子健康患者94例,已排除女方不孕原因和無精子癥,平均年齡31歲.精子計數＞2×1010 L-1的精子健康男性80例,為精子正常組,精子計數＜2×1010 L-1的精子健康男性患者14例,為少精組.同期精液常規檢查完全健康且已有後代的健康男性供精者27例,為健康對照組.方法:所有對象禁欲3～5 d,用手淫法採集精液,經37℃液化後進行精液常規檢查.酶動力學分光光度法測定精子肌痠激酶總活性,瓊脂糖電泳凝膠光密度掃描測定肌痠激酶的同工酶相對含量.主要觀察指標:精子計數,精子存活率,運動精子百分比,精子肌痠激酶總活性和M-肌痠激酶及B-肌痠激酶同工酶的相對含量.結果:納入患者94例和健康對照者27例,均進入結果分析.①少精組的精子計數,精子存活率和運動精子百分比(Ⅱ+Ⅲ,WHO分類法)均顯著低于健康對照組,精子正常組除精子計數外,其他指標也顯著低于健康對照組[健康對照組、精子正常組、少精組精子計數分彆為(6.05±0.81)×1010 L-1,(7.76±1.37)×1010 L-1,(1.46±0.19)×1010 L-1,P＜0.01;健康對照組、精子正常組、少精組精子存活率分彆為85.1%,56.8%,58.2%,P＜0.01;健康對照組、精子正常組、少精組運動精子百分比(Ⅱ+Ⅲ)分彆為62.9%,34.6%,29.5%,P＜0.01].②少精組肌痠激酶活性顯著高于健康對照組(少精組、健康對照組每1×108精子中肌痠激酶活性分彆為(9.000±6.117),(1.933±0.943)kat,P＜0.05),精子正常組每1×108精子中肌痠激酶活性為(2.800±0.862)kat,雖略高于健康對照組,但兩者比較差異無顯著性(P＞0.05).③精子正常組和少精組M-肌痠激酶同工酶含量都顯著減少(健康對照組、精子正常組、少精組分彆為30.5%,19.0%,18.0%,P＜0.05),提示兩組患者的精子分化程度與健康對照組精子分化程度可能有明顯不同.結論:精子肌痠激酶活性測定對少精子癥的診斷和病因分析具有一定的意義.精子M-肌痠激酶同工酶相對含量在不同精子健康男性群體中的分佈規律及其在男性精子健康診斷中的作用有待更深入的研究.
배경:작위포장중적일충생화표지물,인정자중기산격매활성증고여정장잔여량화이상공능정자비례이자유관.타즉시성숙정자적표지,우여수정능력상관.목적:관찰정자기산격매활성급정자M-기산격매、B-기산격매동공매적상대함량재건강생육남성화정자건강남성정자중적차이,평개기재남성정자건강림상진단중적의의.설계:병례-대조관찰.단위:사천성계화생육과학연구소생화실.대상:우1999-01/2000-10선택사천성계화생육과학연구소전과문진수치남성정자건강환자94례,이배제녀방불잉원인화무정자증,평균년령31세.정자계수＞2×1010 L-1적정자건강남성80례,위정자정상조,정자계수＜2×1010 L-1적정자건강남성환자14례,위소정조.동기정액상규검사완전건강차이유후대적건강남성공정자27례,위건강대조조.방법:소유대상금욕3～5 d,용수음법채집정액,경37℃액화후진행정액상규검사.매동역학분광광도법측정정자기산격매총활성,경지당전영응효광밀도소묘측정기산격매적동공매상대함량.주요관찰지표:정자계수,정자존활솔,운동정자백분비,정자기산격매총활성화M-기산격매급B-기산격매동공매적상대함량.결과:납입환자94례화건강대조자27례,균진입결과분석.①소정조적정자계수,정자존활솔화운동정자백분비(Ⅱ+Ⅲ,WHO분류법)균현저저우건강대조조,정자정상조제정자계수외,기타지표야현저저우건강대조조[건강대조조、정자정상조、소정조정자계수분별위(6.05±0.81)×1010 L-1,(7.76±1.37)×1010 L-1,(1.46±0.19)×1010 L-1,P＜0.01;건강대조조、정자정상조、소정조정자존활솔분별위85.1%,56.8%,58.2%,P＜0.01;건강대조조、정자정상조、소정조운동정자백분비(Ⅱ+Ⅲ)분별위62.9%,34.6%,29.5%,P＜0.01].②소정조기산격매활성현저고우건강대조조(소정조、건강대조조매1×108정자중기산격매활성분별위(9.000±6.117),(1.933±0.943)kat,P＜0.05),정자정상조매1×108정자중기산격매활성위(2.800±0.862)kat,수략고우건강대조조,단량자비교차이무현저성(P＞0.05).③정자정상조화소정조M-기산격매동공매함량도현저감소(건강대조조、정자정상조、소정조분별위30.5%,19.0%,18.0%,P＜0.05),제시량조환자적정자분화정도여건강대조조정자분화정도가능유명현불동.결론:정자기산격매활성측정대소정자증적진단화병인분석구유일정적의의.정자M-기산격매동공매상대함량재불동정자건강남성군체중적분포규률급기재남성정자건강진단중적작용유대경심입적연구.
BACKGROUND: As a biochemical marker of cytoplasm, the increased activity of creatine kinase (CK) in human spermatozoa is correlated with both the residual cytoplasma and the ratio of sperm with abnormal func tion. It is a marker of mature sperm and associated with the potential of in semination. OBJECTIVE: To investigate the differences of CK activity in sperm and the relative contents of sperm CK-MM and CK-BB isoenzymes between normal fertile males and infertile males, and evaluate its significance in clinical diagnosis of male infertility. DESIGN: Case-control observation. SETTING: Laboratory of Biochemistry, Family Planning Research Insti tute of Sichuan Province. PARTICIPANTS: Ninety-four male infertile patients between January 1999 and October 2000 were selected from the Department of Family Planning Sciences of Sichuan Province, who had no aspermatism with their wives proved to be fertile. The average age of subjects were 31 years. Eighty subjects with the sperm count ＞ 2×1010 L-1 were taken as normal sperm group and 14 subjects with the sperm counts ＜ 2×1010 L-1 were considered as oligospermia group. Semen obtained from 27 healthy males who were normal in routine examinations and with children was taken as the healthy control group.METHODS: Semen sample collected by masturbation after abstinence of 3 to 5 days was incubated at 37 ℃ for liquefication and routinely analyzed.Total activity of CK in sperm was determined by using a kinetic spectrophotometry and the relative contents of CK isozyme was determined by agarose gel electrophoresis followed by density scanning of CK isozyme.MAIN OUTCOME MEASURES: Sperm counts, percentages of viability and motility of sperm, total CK activity and the relative contents of CK-MM and CK-BB isozyme in spermatozoa.RESULTS: A total of 94 enrolled patients and 27 normal controls were involved in the analysis of results. ①Sperm counts, percentage of viability and motility in oligospermia group ( Ⅱ + Ⅲ, WHO method) were obviously lower than those in the healthy control group, and those in the normal sperm group, except the sperm counts, were remarkably lower than the healthy control group [the sperm counts in healthy control group, normal sperm group and oligospermia group was (6.05 ±0.81 )×1010 L-1, (7.76±1.37)×1010 L-1 and (1.46±0.19)×1010 L-1 respectively (P ＜ 0.01). The survival rate in healthy control group, normal sperm group and oligospermia group was 85.1%,56.8% and 58.2%, P ＜ 0.01, and the sperm motility was 62.9% ,34.6% and 29.5% respectively, P ＜ 0.01].②Total activity of sperm CK in oligospermia group was significantly higher than the healthy control group [respectively (9.000±6.117) and (1.933 ±0.943) kat/108 sperm,P＜ 0.05],although that in the normal sperm group (2.800±0.862) kat/108 sperm was a little higher than the healthy control group, while there were no significant difference between the two groups (P ＞ 0.05).③The relative contents of sperm CK-MM isozyme in the normal sperm group and oligospermia group were obviously decreased (that in the healthy control group, normal sperm group and oligospermia group was 30.5%, 19.0% and 18.0% respectively,P ＜ 0.05), which implied a remarkable difference in sperm differentiation between healthy control group and the latter two groups.CONCLUSION: The determination of sperm CK is meaningful for the diagnosis and pathogenesis of oligospermia. More work should be done on the distribution of sperm CK-MM isozyme in different infertile population as well as its importance in the diagnosis of infertility.
