湖北农业科学
湖北農業科學
호북농업과학
2009年
12期
2926-2928
,共3页
黑木耳%内部简单重复序列分子标记%DNA提取%引物筛选
黑木耳%內部簡單重複序列分子標記%DNA提取%引物篩選
흑목이%내부간단중복서렬분자표기%DNA제취%인물사선
Auricularia auricula%ISSR marker%DNA extraction%primer screening
以14个黑木耳[Auricularia auricla(L.ex Hook)Underw]菌株为试材,对黑木耳总DNA提取方法和ISSR-PCR扩增反应条件及引物进行了筛选.结果表明.改进的SDS抽提法可以在较短时间内提取到高纯度的DNA产物;并筛选出了可产生遗传多样性的15条引物,其中引物ISSR11、ISSR12、ISSR14扩增结果的稳定性好、多态性强.经凝胶电泳比较,引物ISSR14可作为黑木耳PCR-ISSR反应的适宜引物.
以14箇黑木耳[Auricularia auricla(L.ex Hook)Underw]菌株為試材,對黑木耳總DNA提取方法和ISSR-PCR擴增反應條件及引物進行瞭篩選.結果錶明.改進的SDS抽提法可以在較短時間內提取到高純度的DNA產物;併篩選齣瞭可產生遺傳多樣性的15條引物,其中引物ISSR11、ISSR12、ISSR14擴增結果的穩定性好、多態性彊.經凝膠電泳比較,引物ISSR14可作為黑木耳PCR-ISSR反應的適宜引物.
이14개흑목이[Auricularia auricla(L.ex Hook)Underw]균주위시재,대흑목이총DNA제취방법화ISSR-PCR확증반응조건급인물진행료사선.결과표명.개진적SDS추제법가이재교단시간내제취도고순도적DNA산물;병사선출료가산생유전다양성적15조인물,기중인물ISSR11、ISSR12、ISSR14확증결과적은정성호、다태성강.경응효전영비교,인물ISSR14가작위흑목이PCR-ISSR반응적괄의인물.
The methods of DNA extration,primers of ISSR and rection conditions were selected using fourteen kinds of A uricularia auricular ag experiment materials.The results showed that the improved SDS method could obtain pure DNA in short time.Fifteen primers of genetic variation were selected.Primers ISSR11、ISSR12、ISSR14 could produce stable and intense polymorphism.Primer ISSR14 could be used as the suitable primer for A.auricula variety.
