CAJ | 학술논문

目的观察日本血吸虫DNA疫苗pcDNA3/SjSDISP免疫小鼠后可否引起自身免疫性疾病和免疫耐受。方法采用ELISA的方法检测pcDNA3/SjSDISP免疫小鼠后，SjSDISP特异性抗体的效价及自身免疫性抗体(抗核抗体和抗dsDNA抗体)的产生情况，同时通过体重变化及主要脏器病理切片观察质粒DNA的毒性作用。结果以ELISA的方法检测小鼠产生的抗Sj SDISP特异性抗体的效价为1∶400，未检出自身免疫性抗体。实验组与对照组小鼠体重变化无明显差异，组织病理学观察未见异常变化。结论 DNA疫苗pcDNA3/S SDISP对小鼠无诱导自身免疫性疾病和免疫耐受的迹象.
목적 관찰일본혈흡충DNA역묘pcDNA3/SjSDISP면역소서후가부인기자신면역성질병화면역내수。방법 채용ELISA적방법검측pcDNA3/SjSDISP면역소서후，SjSDISP특이성항체적효개급자신면역성항체(항핵항체화항dsDNA항체)적산생정황，동시통과체중변화급주요장기병리절편관찰질립DNA적독성작용。결과 이ELISA적방법검측소서산생적항Sj SDISP특이성항체적효개위1∶400，미검출자신면역성항체。실험조여대조조소서체중변화무명현차이，조직병이학관찰미견이상변화。결론 DNA역묘pcDNA3/S SDISP대소서무유도자신면역성질병화면역내수적적상.
To determine the possibility whether DNA vaccines pcDNA3/SjSDISP of Schistosoma japonicum to induce autoimmunity and immune tolerance in the vaccinated mice, the titer of the specific antibodies against SjSDISP and the production of the autoimmune antibodies, such as presence of anti-nuclear and anti-dsDNA antibodies in the vaccinated mice were detected by ELISA assay and the toxicity of the plasmid DNA was studied through the observation of the change in body weight and the pathological examination of the major organs in mice. It was found that the titer of the specific antibody against SjSDISP was 1∶400 as determined by ELISA, but no autoimmune antibody could detected. The difference of the body weight of mice between the experimental and the control groups was not significant. No abnormal results of histopathological examination were obtained in both groups. From these observations, it is clear that there is no evidence to show that DNA vaccine pcDNA3/SjSDISP can induce auto-immunity and immune tolerance in mice.