CAJ | 학술논문

目的:探讨IL-7对胸腺T细胞及胸腺树突状细胞分化的影响.方法:摘取15～16日龄胎鼠胸腺进行体外器官培养(胚胎胸腺器官培养-FTOC),分别将细胞因子IL-7和培养基滴加在胸腺小叶上,12天后收集不同条件下经FTOC培养获得的胸腺细胞,流式细胞仪检测细胞表面分子CD4、CD8、CD11c、B220、Ia等的表达,通过光学显微镜观察细胞形态,通过细胞计数检测细胞数目的变化.再将经FTOC培养获得的胸腺细胞和异源的T细胞进行混合淋巴细胞反应,通过MTT法检测T细胞的增殖情况.结果:细胞计数结果表明添加外源性IL-7组的胸腺细胞数目明显减少,流式细胞仪检测结果显示其中胸腺CD4~-CD8~-双阴性细胞及CD8~+单阳性细胞比例有所增加,而CD4~+CD8~+双阳性细胞比例显著下降,CD4~+单阳性细胞比例没有明显变化;此外,B细胞和树突状细胞、NK细胞数量均有不同程度的增加.结论:IL-7在胸腺T细胞及胸腺树突状细胞的分化发育中发挥重要的调节作用.
목적:탐토IL-7대흉선T세포급흉선수돌상세포분화적영향.방법:적취15～16일령태서흉선진행체외기관배양(배태흉선기관배양-FTOC),분별장세포인자IL-7화배양기적가재흉선소협상,12천후수집불동조건하경FTOC배양획득적흉선세포,류식세포의검측세포표면분자CD4、CD8、CD11c、B220、Ia등적표체,통과광학현미경관찰세포형태,통과세포계수검측세포수목적변화.재장경FTOC배양획득적흉선세포화이원적T세포진행혼합림파세포반응,통과MTT법검측T세포적증식정황.결과:세포계수결과표명첨가외원성IL-7조적흉선세포수목명현감소,류식세포의검측결과현시기중흉선CD4~-CD8~-쌍음성세포급CD8~+단양성세포비례유소증가,이CD4~+CD8~+쌍양성세포비례현저하강,CD4~+단양성세포비례몰유명현변화;차외,B세포화수돌상세포、NK세포수량균유불동정도적증가.결론:IL-7재흉선T세포급흉선수돌상세포적분화발육중발휘중요적조절작용.
Objective:To investigate the effect of IL-7 on differentiation of thymic T lymphocytes and dendritic cells' differentiation in vitro.Methods:Thymic lobes were apspectically removed from 15-day-old or 16-day-old mouse fetueses and were cultured by the way of fetal thymus organ cultures (FTOC) in vitro.Lobes were cultured in the dishes with or without Interleukin-7(IL-7),then collected the cultured cells respectively.The expression of CD4,CD8,CD11c,B220,Ia,and CD11b was detected by flow cytometry,and the morphology was observed under the ligt microscope.The proliferation of these cells was determined by cell counting.The cultured cells were used as stimulators for allogeneic T cells,whose proliferation were detected later by MTT method.Results:The result of cell counting indicated that the total amount of the thymocytes was decresed obviously after being cultured with IL-7,while the proportion of CD4~+ CD8~+ thymocytes decreased,the proportion of CD4~- CD8~-,and CD4~- CD8~+ thymocytes increased,and no much difference in the amount of CD4~+ CD8~- thymocytes was found.In addition,the amount of B lymphocytes,natural killer cells,and dendritic cells increased in different degree.Conclusion:IL-7 participates in differentiation and development of thymic T lymphocytes and dendritic cells.