CAJ | 학술논문

采用高温热分解前驱体乙酰丙酮铁法合成了尺寸均一且分散性好的油溶性Fe_3O_4纳米粒子, 通过表面配体置换直接将其表面换为水溶性分子聚乙二醇二酸(PEG diacid), 形成了具有表面功能基的稳定无毒的水溶性磁性复合体系. TEM, M-H曲线, FTIR和TGA等表征结果表明, 油溶性Fe_3O_4纳米粒子已成功转移至水相, 且具有很好的顺磁性及生物相容性. 研究结果表明, α-胰凝乳蛋白酶(α-ChT)被成功地固载到水溶性Fe_3O_4纳米粒子表面, 与自由酶相比, 固定化酶的热稳定性明显提高, 并体现出良好的可重复利用性.
채용고온열분해전구체을선병동철법합성료척촌균일차분산성호적유용성Fe_3O_4납미입자, 통과표면배체치환직접장기표면환위수용성분자취을이순이산(PEG diacid), 형성료구유표면공능기적은정무독적수용성자성복합체계. TEM, M-H곡선, FTIR화TGA등표정결과표명, 유용성Fe_3O_4납미입자이성공전이지수상, 차구유흔호적순자성급생물상용성. 연구결과표명, α-이응유단백매(α-ChT)피성공지고재도수용성Fe_3O_4납미입자표면, 여자유매상비, 고정화매적열은정성명현제고, 병체현출량호적가중복이용성.
The oil-soluble magnetic Fe_3O_4 nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)_3 precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.