CAJ | 학술논문

本研究探讨高压氧预处理对同种异体小鼠皮肤移植排斥反应的影响及其分子机制.BALB/c供鼠与C57BI/6受鼠,各自或共同接受高压氧预处理;受鼠在皮肤移植术后,腹腔注射常规免疫抑制剂环孢素A.采用免疫荧光染色技术和流式细胞术,观察不同处理方案对小鼠脾淋巴细胞CD3+、CD4+、CD8+细胞百分率及淋巴细胞表面黏附因子LFA-1(CD11a/CD18)表达的影响.结果表明,与对照组相比,HBO预处理组小鼠脾淋巴细胞CD3+、CD4+、CD8+及其表面黏附分子CD4+CD11a+、CD4+CD18+、CD8+CD11a+、CD8+CD18+的表达均下降,以供鼠和受鼠都接受HBO预处理同时与环孢素A联合应用组下降最明显(P<0.05);HBO预处理与环孢素A联合应用组小鼠一般状态好于单独应用HBO预处理或环孢素A组.结论:高压氧预处理与环孢素A联合应用较单独应用高压氧预处理和环孢素A可更好地降低皮肤移植术后的免疫排斥反应,其保护作用的分子机制与调节淋巴细胞的活性并抑制其表面黏附因子的表达作用相关.
본연구탐토고압양예처리대동충이체소서피부이식배척반응적영향급기분자궤제.BALB/c공서여C57BI/6수서,각자혹공동접수고압양예처리;수서재피부이식술후,복강주사상규면역억제제배포소A.채용면역형광염색기술화류식세포술,관찰불동처리방안대소서비림파세포CD3+、CD4+、CD8+세포백분솔급림파세포표면점부인자LFA-1(CD11a/CD18)표체적영향.결과표명,여대조조상비,HBO예처리조소서비림파세포CD3+、CD4+、CD8+급기표면점부분자CD4+CD11a+、CD4+CD18+、CD8+CD11a+、CD8+CD18+적표체균하강,이공서화수서도접수HBO예처리동시여배포소A연합응용조하강최명현(P<0.05);HBO예처리여배포소A연합응용조소서일반상태호우단독응용HBO예처리혹배포소A조.결론:고압양예처리여배포소A연합응용교단독응용고압양예처리화배포소A가경호지강저피부이식술후적면역배척반응,기보호작용적분자궤제여조절림파세포적활성병억제기표면점부인자적표체작용상관.
The aim of this study was to explore the effect of hyperbaric oxygen(HBO)preconditioning on the rejection of skin allograft in mice and its molecular mechanism.BALB/c donor mice and C57BL/6 recipients received hyperbaric oxygen preconditioning once a day for 7 days.After skin transplantation,the recipients were treated with cyclosporine A(CsA)intraperitoneally.Immunoflurescent staining technique and flow cytometry were used to observe the influence HBO on percentage of spleen lymphocytes CD3+,CD4+,CD8+and cell adhesion molecule LFA-1(CD11a/CD18).The results showed that as compared with control,the numbers of CD3+,CD4+,CD8+,CD4+CD11a+,CD4+CD18+,CD8+CD11a+,CD8+CD18+lymphocytes of spleen decreased in HBO preconditioning groups and CsA group,and decreased markedly in HBO preconditioning combined with CsA group(p < 0.05); the general state of recipien tmice in HBO preconditioning combined with CsA group was better than that of recipient mice received HBO preconditioning or CsA only.It is concluded that the method of HBO preconditioning combined with traditional immunosuppressive agent CsA has remarkable advantage in inhibiting the rejection of skin graft.Its molecular protective mechanism is correlated with the expression of adhesive molecules on T cell subsets.