CAJ | 학술논문

目的探讨肝癌组织及其癌旁组织中RUNX3mRNA和蛋白表达情况,并分析其与临床病理因素的相关性.方法采用RT-PCR(逆转录-聚合酶链反应)和IHC(免疫组织化学)分别检测肝癌组织及其癌旁组织中RUNX3mRNA和蛋白表达水平,并分析与临床病理因素的相关性.结果在51例肝癌组织中RUNX3mRNA表达量相对值为:0.4509±0.0963；在51例相对应的癌旁组织中RUNX3mRNA表达量相对值为:0.9147 ±0.0222；两者间差异有统计学意义(t=33.6087,P＜0.001).在51例肝癌组织中RUNX3蛋白表达阳性率为49.02％( 25/51)；而在51例相对应的癌旁组织中RUNX3蛋白表达阳性率为82.35％ (42/51)；两者间差异有统计学意义(x2=12.5706,P＜0.005).RUNX3mRNA和蛋白表达水平均在分化程度、门静脉癌栓、肝内转移等病理因素差异有统计学意义(P＜0.05),而在性别、肿瘤直径、肿瘤部位、癌肿出血坏死、组织分型的差异均无统计学意义(P＞0.05).结论 RUNX3基因在肝癌组织中mRNA和蛋白表达水平均显著低于在癌旁组织中的表达水平,RUNX3mRNA和蛋白表达下调可能在肝癌的发生、发展过程中起着重要作用；RUNX3基因可能是肝癌的一种抑癌基因.
목적 탐토간암조직급기암방조직중RUNX3mRNA화단백표체정황,병분석기여림상병리인소적상관성.방법 채용RT-PCR(역전록-취합매련반응)화IHC(면역조직화학)분별검측간암조직급기암방조직중RUNX3mRNA화단백표체수평,병분석여림상병리인소적상관성.결과재51례간암조직중RUNX3mRNA표체량상대치위:0.4509±0.0963；재51례상대응적암방조직중RUNX3mRNA표체량상대치위:0.9147 ±0.0222；량자간차이유통계학의의(t=33.6087,P＜0.001).재51례간암조직중RUNX3단백표체양성솔위49.02％( 25/51)；이재51례상대응적암방조직중RUNX3단백표체양성솔위82.35％ (42/51)；량자간차이유통계학의의(x2=12.5706,P＜0.005).RUNX3mRNA화단백표체수평균재분화정도、문정맥암전、간내전이등병리인소차이유통계학의의(P＜0.05),이재성별、종류직경、종류부위、암종출혈배사、조직분형적차이균무통계학의의(P＞0.05).결론 RUNX3기인재간암조직중mRNA화단백표체수평균현저저우재암방조직중적표체수평,RUNX3mRNA화단백표체하조가능재간암적발생、발전과정중기착중요작용；RUNX3기인가능시간암적일충억암기인.
Objective To investigate the expression of RUNX3 mRNA and protein in hepatic cell carcinoma (HCC)and surrounding normal tissue,to analyze the relationship between RUNX3 expression and clinical pathological parameters.Methods Reverse transcription polymerase chain reaction ( RT-PCR ) and immunohistochemistry(IHC) were performed to detect the expression of RUNX3 mRNA and protein in HCC and surrounding normal tissue respectively,and their relationship with clinical pathological parameters were analyzed.Results The relative expression value of RUNX3 mRNA found in 51 cases HCC was 0.4509 ± 0.0963,and that did in 51 cases surrounding normal tissue was 0.9147 ±0.0222.The difference of RUNX3 mRNA expression between two kinds of samples was statistically significant( t =33.6087,P ＜ 0.001 ).The positives rate of RUNX3 protein expression found in 51 cases HCC tissue was 49.02％ (25/51)and that did in 51 cases surrounding normal tissue was 82.35％ (42/51).The difference of RUNX3 protein expression between two kinds of samples was statistically significant ( x2 =12.5706,P ＜ 0.005 ).The difference of RUNX3 mRNA and protein expression in some clinical pathological parameters involving differentiation degree,invasion,cancer thrombus and diversion in liver were statistically significant (P ＜ 0.05 ).However that were not in another clinical pathological parameters involving gender,cancer diameter,cancer location as well as hemorrhage and necrosis of cancer,histotype ( P ＞ 0.05 ).Conclusion The expression of RUNX3 mRNA and protein in HCC were significantly lower than that in surrounding normal tissue. The lower expression of runx3 protein in the HCC probably plays an important role in the tumorigenesis and development of HCC.The RUNX3 gene may be an anti-oncogene of HCC.