CAJ | 학술논문

目的以不同组成丙交酯/乙交酯共聚物(PLGA)为载体,制备载牛血清白蛋白(BSA)聚合物微球,考察共聚物组成、浓度及混合聚乙二醇(PEG)对聚合物微球粒径、BSA包封率和体外释放规律的影响. 方法采用水/油/水(W/O/W)双乳化溶剂挥发技术制备载BSA的PLGA/PEG聚合物微球.利用扫描电子显微镜观察聚合物微球表面形貌,激光动态粒度分析仪测定聚合物微球粒径,紫外分光光度法测定聚合物微球药物包封率和体外释放速率. 结果 PLGA共聚物中GA含量越高,聚合物微球粒径越大.释放速率越快.同时,PEG的加入导致聚合物微球对BSA的包封率增加.最高达88.22%.且释放速率也加快.采用双乳化溶剂挥发法制备的BSA-PLGA/PEG聚合物微球包封率较高、突释量较小、缓慢释放时间达3周以上并能维持较高药物浓度. 结论通过调整各组份的比例及添加PEG,可以得到较高包封率和适当载药量的BSA-PLGA/PEG缓释微球.
목적 이불동조성병교지/을교지공취물(PLGA)위재체,제비재우혈청백단백(BSA)취합물미구,고찰공취물조성、농도급혼합취을이순(PEG)대취합물미구립경、BSA포봉솔화체외석방규률적영향. 방법 채용수/유/수(W/O/W)쌍유화용제휘발기술제비재BSA적PLGA/PEG취합물미구.이용소묘전자현미경관찰취합물미구표면형모,격광동태립도분석의측정취합물미구립경,자외분광광도법측정취합물미구약물포봉솔화체외석방속솔. 결과 PLGA공취물중GA함량월고,취합물미구립경월대.석방속솔월쾌.동시,PEG적가입도치취합물미구대BSA적포봉솔증가.최고체88.22%.차석방속솔야가쾌.채용쌍유화용제휘발법제비적BSA-PLGA/PEG취합물미구포봉솔교고、돌석량교소、완만석방시간체3주이상병능유지교고약물농도. 결론 통과조정각조빈적비례급첨가PEG,가이득도교고포봉솔화괄당재약량적BSA-PLGA/PEG완석미구.
Objective To prepare PLGA/PEG microsphcres for sustained release of bovine serum albumin (BSA), and evaluate the effect of the micrnsphere composition, proportion of the components and integration of PEG on the size, BSA encapsulation rate and in vitro BSA release properties of the microspheres. Methods BSA-loaded PLGA/PEG microspheres were prepared by water-in-oil-in-water double emulsions-solvent evaporation method (W/O/W). The morphology of the microspheres was observed with scanning elect'on microscope, the diameter was determined by Mastersizer 2000, and the encapsulation efficiency and in vitro BSA release were evaluated by UV spectrophotometry. Results Increased GA to LA ratio was associated with increased size of the microspheres and accelerated BSA release. The incorporation of PEG obviously increased the BSA encapsulation rate to as much as 88.22% with also increased BSA release rate. The BSA-PLGA/PEG biodegradable microspheres prepared was characterized by high encapsulation rate, low burst release, and slow BSA release for over 3 weeks at high drug concentrations. Conclusion BSA-PLGA/PEG microspheres with relatively high encapsulation efficiency and proper drug loading can be prepared by adjusting the parameters during the preparation process.