CAJ | 학술논문

背景:目前已有大量文献证实了神经功能指数评价鼠坐骨神经损伤后功能恢复程度的可靠性,且经常被用来评价大鼠坐骨神经及其分支损伤后功能恢复的程度.目的:本文拟用神经功能指数评价不同腓神经修复方式对神经功能的影响.设计:随机对照的研究.地点和材料:实验在北京大学医学部动物实验室完成,所需的实验材料为20只SD鼠.方法:将大鼠随机分为两组,两组中作为支持神经的胫神经均先行去外膜的"开窗"术,第1组行腓神经与胫神经"开窗"端侧缝合,第2组行腓神经与胫神经"开窗"的侧侧缝合.分别在术后的3,6,11,16周通过足印分析法计算出这两组的腓神经功能指数(peroneal nervefunctional index,PFI),用t检验比较两组功能指数的差异.主要观察指标:术后不同时间点两实验组PFI的差异.结果:术后6周:第1组PFI为-39.92±11.67,第2组PFI为-64.49±31.31,差异有显著性意义(t=-2.315,P=0.033);术后16周:第1组PFI为-27.43±12.54,第2组PFI为-15.66±9.81,差异有显著性意义(t=2.314,P=0.048).结论:腓神经术后16周鼠类腓神经与胫神经侧侧缝合修复法功能恢复较端侧缝合法优.
배경:목전이유대량문헌증실료신경공능지수평개서좌골신경손상후공능회복정도적가고성,차경상피용래평개대서좌골신경급기분지손상후공능회복적정도.목적:본문의용신경공능지수평개불동비신경수복방식대신경공능적영향.설계:수궤대조적연구.지점화재료:실험재북경대학의학부동물실험실완성,소수적실험재료위20지SD서.방법:장대서수궤분위량조,량조중작위지지신경적경신경균선행거외막적"개창"술,제1조행비신경여경신경"개창"단측봉합,제2조행비신경여경신경"개창"적측측봉합.분별재술후적3,6,11,16주통과족인분석법계산출저량조적비신경공능지수(peroneal nervefunctional index,PFI),용t검험비교량조공능지수적차이.주요관찰지표:술후불동시간점량실험조PFI적차이.결과:술후6주:제1조PFI위-39.92±11.67,제2조PFI위-64.49±31.31,차이유현저성의의(t=-2.315,P=0.033);술후16주:제1조PFI위-27.43±12.54,제2조PFI위-15.66±9.81,차이유현저성의의(t=2.314,P=0.048).결론:비신경술후16주서류비신경여경신경측측봉합수복법공능회복교단측봉합법우.
BACKGROUND: The reliability of nerve function index(NFI) for evaluating functional recovery following rat sciatic nerve injury has been confirmed by many researches; moreover, NFI has been commonly used to assess functional recovery after sciatic nerve injury and its branches injuries in rat model.OBJECTIVE: To assess the effect of different peroneal neurorrhaphy on nerve function recovery by NFI.DESIGN: A randomised case-control study.SETTINGS and MATERIALS: The experiment was conducted at the Experimental Animal laboratory of Peking University Health Siences Center. Totally twenty SD rats were used.METHODS: All rats were randomly divided into two groups after removing a small epineural window at tibial nerve (TN) that used as donor nerve. The distal end of PN was sutured to the lateral face of the TN in group A, while PN and TN were reinnervated by side -to-side neurorraphy in group B. Footprint analysis was used to calculate Peroneal nerve function index(NFI) of the two groups at 3, 6, 11, 16 weeks after operation,and the difference of PFI between two groups was compared with t test.MAIN OUTCOME MEASURES: The difference of PFI between two groups at different postoperative time points.RESULTS: PFI was - 39. 92 + 11.67 in group A at 6th week postoperatively, significantly different from -64.49 +31.31 of group B, (t = -2. 315,P = 0. 033) PFI was - 27.43 ± 12.54 in group A and - 15.66 ± 9.81 in group B at 16 weeks postoperatively, with significant difference( t = 2. 314,P=0. 048).CONCLUSION: Side-to-side reinnervation by PN and TN was superior to distallateral neurorraphy for nerve functional recovery at 16 weeks after PN operation in rat models.