CAJ | 학술논문

采用Plackett-Bumaan设计、最陡爬坡试验与响应面设计相结合的方法优化达托霉素发酵培养基组成,利用Design Expert 7.0软件设计试验并分析数据.结果表明,培养基中的糊精、酵母粉、酪蛋白水解物是影响达托霉素产量的主要因素.优化后的培养基组成/g·L~(-1)为:糊精10.6,酵母粉1.6,酪蛋白水解物1.3,硫酸钾8,L-门冬氨酸1.5;pH 6.5.在此条件下,达托霉素产量为37.16 me,/L.进一步优化前体癸酸的添加量,最终达托霉素产量达46.54 mg/L.
채용Plackett-Bumaan설계、최두파파시험여향응면설계상결합적방법우화체탁매소발효배양기조성,이용Design Expert 7.0연건설계시험병분석수거.결과표명,배양기중적호정、효모분、락단백수해물시영향체탁매소산량적주요인소.우화후적배양기조성/g·L~(-1)위:호정10.6,효모분1.6,락단백수해물1.3,류산갑8,L-문동안산1.5;pH 6.5.재차조건하,체탁매소산량위37.16 me,/L.진일보우화전체계산적첨가량,최종체탁매소산량체46.54 mg/L.
Plackett-Burman design combined with the path of steepest ascent method and response surface methodology was used for the optimization of the culture medium for daptomycin fermentation. Experimental design and data analysis were obtained by Design Expert 7.0. The results indicated that the major factors on daptomycin fermentation were dextrin, yeast extract and casamino acid. The optimized composition (g·L~(-1)) of the culture medium was dextrin 10.6, yeast extract 1.6, casamino acid 1.3, K_2SO_4 8,L-aspartic acid 1.5; pH 6.5. Under the optimized condition, the yield of daptomycin was 37.16 tng/L. After optimization of the addition of its precursor, the yield of daptomycin could reach to 46.54 mg/L.