CAJ | 학술논문

为了考察红色诺卡氏菌细胞壁骨架(Nocardia rubra cell wall skeleton,N-CWS)灌胃给药对小鼠的体内抑瘤效应及其免疫调节作用,采用小鼠肉瘤S_(180)的移植性肿瘤模型,检测N-CWS灌胃给药的抑瘤活性;同时观察N-CWS体内细胞毒作用和对正常小鼠的毒性、免疫器官重量、巨噬细胞(MΦ)吞噬功能及脾淋巴细胞转化的影响.结果显示,小鼠灌服N-CWS的LD_(50)＞1.2 g/kg;N-CWS 200、400、800 mg/kg剂量灌胃小鼠对S_(180)有明显的抑制作用,其抑瘤率分别为63.33%、71.11%、64.88%;与对照组比较,N-CWS可增加免疫器官重量和提升外周血白细胞数量、能明显提高小鼠MΦ的吞噬活性及显著提高淋巴细胞转化率(P＜0.05),同时N-CWS激活了的MΦ对肿瘤细胞的细胞毒效应亦明显增强(P＜0.05).因此N-CWS适用于口服给药,对小鼠移植性肿瘤有明显的抑制作用,其抗肿瘤作用可能与增强机体免疫功能有关.
위료고찰홍색낙잡씨균세포벽골가(Nocardia rubra cell wall skeleton,N-CWS)관위급약대소서적체내억류효응급기면역조절작용,채용소서육류S_(180)적이식성종류모형,검측N-CWS관위급약적억류활성;동시관찰N-CWS체내세포독작용화대정상소서적독성、면역기관중량、거서세포(MΦ)탄서공능급비림파세포전화적영향.결과현시,소서관복N-CWS적LD_(50)＞1.2 g/kg;N-CWS 200、400、800 mg/kg제량관위소서대S_(180)유명현적억제작용,기억류솔분별위63.33%、71.11%、64.88%;여대조조비교,N-CWS가증가면역기관중량화제승외주혈백세포수량、능명현제고소서MΦ적탄서활성급현저제고림파세포전화솔(P＜0.05),동시N-CWS격활료적MΦ대종류세포적세포독효응역명현증강(P＜0.05).인차N-CWS괄용우구복급약,대소서이식성종류유명현적억제작용,기항종류작용가능여증강궤체면역공능유관.
It was studied the effect of Nocardia rubra cell wall skeleton(N-cws)on transplanted tumor and immunological function in mice given in oral administration.Transplanted tumor model of sarcoma 180 in mice was used to evaluate the antitumor effect of N-CWS.Meanwhile the immune organ weights,the phagocytosis of peritoneal macrophage and the lymphocyte transformation were determined in normal mice.After oral administration of N-CWS 200,400,800 mg/kg to mice,the inhibition rates on sarcoma 180 were 63.33%,71.11%,64.88%.The medial lethal dose(LD_(50))of N-CWS was more than 1.2 g/kg.Compared with control group,the spleen and thymus weights in normal mice were increased obvioualy(P＜0.05).the lymphocyte transformation and the phagocytosis of peritoneal maerophage from mice were also increased(P＜0.05).In conclusion,N-CWS could bc siren via oral administration.It has the antineoplastic effect,partially,by enhancing immunological function.