CAJ | 학술논문

桑树杂交组合抗青枯病能力鉴定及与抗病相关酶活性的研究
상수잡교조합항청고병능력감정급여항병상관매활성적연구
Evaluation of Resistance Against Bacterial Wilt and Investigation on Activity of Enzymes Related to Disease Resistance in Mulberry Hy-bridized Combinations

万方数据

蚕业科学蠶業科學 잠업과학
ACTA SERICOLOGICA SINICA
2010年 2期 300-303 ,共4页

罗国庆%唐翠明%王振江%吴福泉%吴剑安%杨琼%陈列辉%钟建武%黄炳辉%肖阳%刘新涛%邢东旭

라국경%당취명%왕진강%오복천%오검안%양경%진렬휘%종건무%황병휘%초양%류신도%형동욱

桑树%杂交组合%青枯病%抗性鉴定%过氧化物酶桑樹%雜交組閤%青枯病%抗性鑒定%過氧化物酶
상수%잡교조합%청고병%항성감정%과양화물매
Morus L.%Hybridized combination%Bacterial wilt%Resistance evaluation%Peroxidase

利用抗青枯病能力较强的桑树资源人工组配了24个杂交组合,并鉴定其抗病能力;对具有不同抗性水平的杂交组合的叶片过氧化物酶(POD)、多酚氧化物酶(PPO)和苯丙氨酸解氨酶(PAL)活性进行比较分析.抗病能力鉴定结果表明,24个桑树杂交组合对青枯病的抗性存在明显的差异,其中04-19×抗10和69×98-8为2个强抗组合.酶活性测定结果表明,桑树叶片中的POD活性与桑树杂交组合抗青枯病能力有密切关系,强抗性杂交组合的POD活性明显高于感病杂交组合.可将桑树叶片中的POD酶活性作为生化遗传标记之一,并结合常规抗病鉴定方法应用于桑树抗青枯病品种的杂交亲本选择.
이용항청고병능력교강적상수자원인공조배료24개잡교조합,병감정기항병능력;대구유불동항성수평적잡교조합적협편과양화물매(POD)、다분양화물매(PPO)화분병안산해안매(PAL)활성진행비교분석.항병능력감정결과표명,24개상수잡교조합대청고병적항성존재명현적차이,기중04-19×항10화69×98-8위2개강항조합.매활성측정결과표명,상수협편중적POD활성여상수잡교조합항청고병능력유밀절관계,강항성잡교조합적POD활성명현고우감병잡교조합.가장상수협편중적POD매활성작위생화유전표기지일,병결합상규항병감정방법응용우상수항청고병품충적잡교친본선택.
24 hybridized combinations were prepared using mulberry germplasms with relatively high resistance against bacterial wilt and evaluated on their disease resistance.Moreover,activity of peroxidase(POD),polyphenol oxidase (PPO)and phenylalanine ammonia lyase(PAL)in Ieaves of the hybridized combinations with different resistance levels were compared and analyzed.Results of disease resistance evaluation indicated that the resistance of 24 mulberry hy-bridized combinations was significantly varied.Among them.04-19×Kang 10 and 69×98-8 were the two combinations with high resistance.Results of enzvme activity analysis revealed that POD activity jn mulberry Ieaves was closely reIaled to resistance of the hybridized combinations against bacterial wilt.The hybridized combinations with high resistance had remarkably higher POD activity over the susceptible hybridized combinations.It was suggested to use POD activity as one of the biochemicaI genetic markers for cross parental selection in breeding mulberry varieties resistant to bacterial wilt together with other oonventionaI methods on evaluating disease resistance.