CAJ | 학술논문

[目的]对农杆菌介导转化获得的稻瘟病抗性基因Pi-d2的水稻植株稳定后代的主要农艺性状和米质进行了分析,以期为转基因水稻的生态学和安全性评价提供的依据。[方法]在田间调查了转Pi-d2基因水稻植株的主要农艺性状,并对转基因水稻稻米理化特性和氨基酸含量进行测定。[结果]外源基因Pi-d2的导入可引起受体植株后代农艺性状可遗传的变异。大部分转基因植株生长正常,整齐一致,与对照相比,一半左右植株株高变高或矮。分蘖数、穗长、有效穗、结实率和千粒重与对照无明显差异,少部分表现增加或减少的趋势。稻米的氨基酸测定结果表明转抗病基因水稻改变了原亲本的总氨基酸含量,呈下降趋势,但淀粉含量、胶稠度和糊化温度并未改变。[结论]转Pi-d2基因入水稻中对植株株高影响明显,但对稻米的理化特性影响不大。
[목적]대농간균개도전화획득적도온병항성기인Pi-d2적수도식주은정후대적주요농예성상화미질진행료분석,이기위전기인수도적생태학화안전성평개제공적의거。[방법]재전간조사료전Pi-d2기인수도식주적주요농예성상,병대전기인수도도미이화특성화안기산함량진행측정。[결과]외원기인Pi-d2적도입가인기수체식주후대농예성상가유전적변이。대부분전기인식주생장정상,정제일치,여대조상비,일반좌우식주주고변고혹왜。분얼수、수장、유효수、결실솔화천립중여대조무명현차이,소부분표현증가혹감소적추세。도미적안기산측정결과표명전항병기인수도개변료원친본적총안기산함량,정하강추세,단정분함량、효주도화호화온도병미개변。[결론]전Pi-d2기인입수도중대식주주고영향명현,단대도미적이화특성영향불대。
[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice were observed in field experiment and the grain chemical characters and amino acid content were measured.[Results] Introduction of foreign gene Pi-d2 resulted in stably hereditable variation in agronomic characteristics in the descents.Most of the transgenic lines grew normally and orderly.Compared with the control（wild type plants）,about half of transgenic plants showed an increased or reduced plant height.There was no observable difference between transgenic plants and controls in tiller number,length of panicle,panicles per plant,seed-setting rate and 1 000-grain weight.Total amino acid content in transgenic rice was reduced,while the starch content,GC and GT were not altered in comparison with the control.[Conclusion] Introduction of foreign gene Pi-d2 has remarkable influence on plant height,while little on grain chemical characters.