CAJ | 학술논문

目的探讨人类纤溶酶原激活物抑制物-1(PAI-1)基因4G/5G多态性与2型糖尿病(T2DM)患者内皮依赖性血管舒张功能(EDF)的关系.方法采用特异性多聚酶链反应技术检测66例T2DM患者和33例健康对照组PAI-1基因4G/5G多态性;利用B型超声检测T2DM患者EDF;同时检测PAI-1和纤维蛋白原.结果 T2DM患者与健康对照组基因型分布频率均符合Hardy-Weinberg平衡,差异无统计学意义(P0.05).T2DM患者比健康对照组具有更高的PAI-1、纤维蛋白原水平.T2DM患者PAI-1基因4G/4G型PAI-1水平明显高于其他两型,EDF明显低于其他两型(P<0.05).结论 PAI-1基因多态性可能影响T2DM患者的EDF.
목적 탐토인류섬용매원격활물억제물-1(PAI-1)기인4G/5G다태성여2형당뇨병(T2DM)환자내피의뢰성혈관서장공능(EDF)적관계.방법 채용특이성다취매련반응기술검측66례T2DM환자화33례건강대조조PAI-1기인4G/5G다태성;이용B형초성검측T2DM환자EDF;동시검측PAI-1화섬유단백원.결과 T2DM환자여건강대조조기인형분포빈솔균부합Hardy-Weinberg평형,차이무통계학의의(P0.05).T2DM환자비건강대조조구유경고적PAI-1、섬유단백원수평.T2DM환자PAI-1기인4G/4G형PAI-1수평명현고우기타량형,EDF명현저우기타량형(P<0.05).결론 PAI-1기인다태성가능영향T2DM환자적EDF.
Objective To investigate the relationship between the polymorphisms of plasminogen activator inhibitor-1(PAI-1)gene and the level of endothelium-dependent vasodilatation (EDF) in patients with type 2 diabe- tes(T2DM).Methods The polymorphisms of PAI-1(4G/5G)gene were determined by polymerase chain reaction (PCR)and the EDF was assessed by non-invasive hish resolution B-mode ultrasonography in 66 T2DM patients and 33 controls.Plasma plasminogen activator inhibitor type-1(PAI-1)and fibrinogen(Fg)were measured.Results Frequencies of PAI-1 alleles and genotypes in T2DM patients and controls were all in accordance with the Hardy Weinberge quilibrium,without significant differences between T2DM patients and controls(P>0.05).PAI-1 and Fg were significantly higher in T2DM patients than in controls.There were significant differences in EDF and PAI- lamong different genotypes of PAI-1(P<0.05).Conclusion The PAI-1 genotype may affect EDF in T2DM pa- tients.